FastQCFastQC Report
Sat 24 Jun 2023
ERR2812371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154172614
Total Bases15.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT33955652.202443684323858No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT32359332.098902597578063No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG22749131.4755623200369425No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA18511091.2006730326308148No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC14131480.9166011805442957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA13339120.8652068388747692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT11469340.7439284904386456Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG10776540.698991845594575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8677510.5628438005208889No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC7584320.49193691429529757No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC7212150.4677970887877661No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACGTC7006850.45448084573567654No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6214590.4030929903024152No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6019540.3904415864674903No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACGT5793590.3757859356266736No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG5380740.34900750920653134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4739900.3074411127257659Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4684020.3038166039008718Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT4400420.28542163785327007No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4363990.2830587019819227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA4239660.27499436443362113No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC4030400.2614212664254366Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3941900.2556809473308924No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3773870.24478212453477632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC3712640.2408106020697035Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC3563290.23112340820789354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA3310490.21472620292991856Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3260050.21145454535784156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC3231800.20962218361297294No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCT3220320.2088775636897484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG3100920.20113299758931247No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC3043620.197416384209455No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2940930.19075566818890416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2799650.18159191359368143Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2704860.17544361023806732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2480980.1609222244879366No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC2467660.16005825781743574No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC2415830.15669644156127494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT2244860.14560692341896725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2109920.13685439620294693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2055200.13330512771872702No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC1934160.1254541873435447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1896000.12297903958481239No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC1888670.12250359846658629No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1867730.12114538059269074Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1848970.11992856266937264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGCCGGAGATCGGAAGAGCACACGTCTGA1819370.11800863673492622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGATGGTAGTGGGTTATCAAGATCGGAAGAGCACACGTCTGAA1791170.11617951810819008Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1772910.11499513136619711Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG1766440.11457547187985019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG1716370.11132781338195383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1713310.11112933455224416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC1687390.10944810211235051No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1681140.10904271234578665No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1633140.10592931893857621No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1600130.10378821234749254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1589450.1030954823143882Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)