FastQCFastQC Report
Sat 24 Jun 2023
ERR2812372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68224044
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC11212751.6435188157418519No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7097521.0403253140491056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4463480.6542385555450216No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3852030.5646147273239915No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2447860.35879725921846556No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2304290.3377533586252964No Hit
ACACCTGCATTAAAAATTTTGGTTGGGAGATCGGAAGAGCACACGTCTGA2131480.31242357899511203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2099810.307781520544282No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1631510.2391400310424284No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1583520.2321058540593108No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1551170.22736412400296882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1520010.2227968192562728Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1509140.22120353932698567No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1326040.19436549378398033No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1183640.17349308698264793No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1168800.1713179007682394No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT1090280.15980876184941484No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1063070.15582043187003103No Hit
CGTGAAACCTATGGTGAAATGGCTGACTGAGATCGGAAGAGCACACGTCT1036650.15194789684410967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1010870.14816917038808194No Hit
TCCAAGTTAGTGACAGATCTTACCAAAGTAGATCGGAAGAGCACACGTCT1009320.14794197775787082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC1001210.14675324728625No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC990190.1451379809733941No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC953630.13977916641822052No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG944470.13843653126161798No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA923500.13536283483869704No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC911070.13354089652029422No Hit
AGACCAACCAAGATGAGTGCTCTGGGAGAGATCGGAAGAGCACACGTCTG904560.13258668747340746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC883740.12953497743405537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC823530.12070964306953132No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC807420.11834830547424013No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAG769200.112746174940905No Hit
ACATAAAGCACCTGGCTTACACCCAGAAAAAGATCGGAAGAGCACACGTC761040.11155011567476124No Hit
GTGATGTGCACTGCTTTTCATGATAATGAAGATCGGAAGAGCACACGTCT758110.11112064831571697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC711870.10434297914090229No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC686350.10060236241639384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)