FastQCFastQC Report
Sat 24 Jun 2023
ERR2812376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190808064
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC103157135.406329682166891No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT55200892.8930061362605723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA52216322.7365887429160227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG48664432.5504388535696267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT47774322.503789357665722No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA46538952.439045238675028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG44682432.341747464090407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT37241721.951789626669028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA25685521.3461443642130346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC23089991.2101160462484435Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA19980721.047163289702473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA18053670.9461691304619076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT17669660.9260436707748368No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC12934900.6779011184768375No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC12787890.6701965174805191No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC11599820.6079313293593294No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG10857920.5690493248754938No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG9779840.5125485681779152No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT9206910.4825220594450348Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG9174270.48081143991901726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG8535680.44734377683324744No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG7412000.38845318403314444Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT6853360.3591755954297613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT6290110.32965640278180275No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT5561070.2914483740058282No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5214560.27328824006096514No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA5151470.269981776032275No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT5040460.2641638877484759No Hit
CTCCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA4924490.2580860523798407Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCACAACCGCGCTTGACTAGCTTGACGTTTAGATCGGAAGAGCACACG4879070.25570564984087885No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4805070.25182740704292245No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4281130.2243683998596621No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC4074710.21355019880082218No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC4022280.21080241136978364Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3983030.20874537042627297No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACGT3953960.2072218499109136No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC3953830.20721503678167397No Hit
CCGCTGGTGTAGTGGTATCATGTAAGATTAGATCGGAAGAGCACACGTCT3745440.19629359061051005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCA3635120.1905118643203675No Hit
GTAAGTGACGATAAAGTGTGTCTGAGGAGATCGGAAGAGCACACGTCTGA3598680.1886020917858063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC3494500.1831421548305212No Hit
ACACCTGCATTAAAAATTTTGGTTGGGAGATCGGAAGAGCACACGTCTGA3302220.1730650125982097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG3239220.16976326535130087No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG3234440.16951275183002748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT3195640.1674792947954233No Hit
CTGGTGTAGTGGTATCATGTAAGATTAGATCGGAAGAGCACACGTCTGAA3060490.16039626082050706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3018930.1582181558112764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2757940.14454001273237593No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2735600.14336920267688477No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2662870.13955751891073115No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2644390.13858900638497124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT2619390.13727878922349948No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2509640.1315269358846385No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2423290.12700144580891506No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC2286610.11983822654371673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2236590.11721674404704405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2218160.11625085195560707No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2058830.10790057594211531No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1967350.1031062293048579No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1944230.10189454047392882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)