FastQCFastQC Report
Sat 23 Sept 2023
ERR2812378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170764399
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA75686694.432228874591126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC53728473.146350780059256No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC44634342.613796567749464No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA36396682.131397423183037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT34233032.0046936129819426No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT20305141.1890733735431587No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG18889361.1061649916854157No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT18049231.056966797862826No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC16100450.9428458211597138No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG14315080.8382941692665109No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT12106270.7089457797348029No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC10738910.6288728835101045No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG9397430.5503155256617628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG8151460.4773512539929356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8095910.4740982340235918No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA7788500.45609623818604017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT7086880.4150092198081639No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC6824890.3996670289572477No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT6600650.3865354862403141No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT6284410.3680164037001647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6022380.3526718704406297No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC5907680.345955013726251No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG5894440.3451796764734317No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG5874310.3440008593360259No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG5365790.31422181856535564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG5302580.3105202273455136No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC5132890.3005831443824541No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4726270.27677138956814995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC4647840.2721785118688586No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4581630.26830124000260736No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC4448220.2604887216567898No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4147580.24288317847796834No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC3981000.2331282177850197Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC3929400.2301065106667813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA3913690.22918652968175177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3610380.21142463072762607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3610040.21140472025436638Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT3350930.19623118282400304No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCA3334720.19528192173123862No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3227060.1889773289337668No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3000220.17569352965661186No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2985910.17485553297323994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC2748500.16095275221856986No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAG2695850.15786955687408824No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGC2675270.15666438763972107No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2515040.14728128431500526No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG2501530.14649013580400913No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC2466050.1444124193591429No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG2408270.14102881010930152No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA2383740.1395923280238289No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2271300.13300781739641176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2220550.13003588646132266No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAA2137580.12517714538379865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2094720.12266725454876574No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC2048670.11997055662638441No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2020120.11829866247472344No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCAC2017260.11813118025847999No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1979920.11594454181284004Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1901310.11134112327476407No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1865790.10926106442127904No Hit
GTAAGTGACGATAAAGTGTGTCTGAGGAGATCGGAAGAGCACACGTCTGA1861850.1090303371723283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCACA1856760.10873226567558733No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1833320.10735961422497671No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC1821080.10664283718762714No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG1717060.1005514035744652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)