FastQCFastQC Report
Fri 23 Jun 2023
ERR2812382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125913653
Total Bases12.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG40071443.18245393134611TruSeq Adapter, Index 6 (100% over 49bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA36988312.9375932727485874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT33494022.6600784904556773No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC19217791.526267369909441No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT12166750.9662772630383457No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA9636080.7652927041994406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG8278930.657508522924039No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT7598660.6034818162252825No Hit
ATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGA6803080.5402972463994831Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA6475030.5142436777686055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6353450.5045878543449136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT6224480.4943451207789198No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA6201890.49255103415989365No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG5447670.43265125506286434Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG5028280.3993435088409356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC4970250.3947347949630213Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAA4627570.36751931897329676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC4438320.3524891776430313No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT4356720.34600854603114406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT4255910.3380022657272917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTGA4087380.3246176965416133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC3931830.31226399253145326No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3871090.3074400517948598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3831240.3042751845187114Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC3507800.27858773980610346No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC3474210.27592003863155334No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAAAGATCGGAAGAGCACACGTCTGAA3333480.26474333168619923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3315360.2633042502547361Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3230250.2565448561801317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA3206500.25465864293525026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT3122050.24795166573397723No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT3017630.2396586810169029No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA3016440.2395641718058962No Hit
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC2928800.23260384638352122No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC2916470.2316246038862839No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC2902260.23049605271955692No Hit
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT2764510.21955601589924487Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG2703170.21468442345962277No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2525040.20053742702548705No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2512990.19958042198966303No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA2493430.1980269764709312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2474600.19653150719088422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2398150.1904598860299923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC2270400.1803140442601566No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG2131230.16926123174267688No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2051110.16289814099826014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1954360.15521430388490118Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1948510.1547496997803725No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1921820.15262999319065107No Hit
ACATATACTAAAATTGGAACGATACAGAGAGATCGGAAGAGCACACGTCT1905070.15129971648110313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1850380.1469562637500478No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1814220.14408445444752524No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1748910.138897566572864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1729150.1373282371531227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1637460.1300462627353048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1587210.126055432606661No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1541030.1223878398635611Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1532370.12170006694984856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAA1488230.11819448999704582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1484020.11786013388079529Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGA1477960.1173788516802066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTAGGTAGAGGTGAAAAGCCTAAGATCGGAAGAGCACACGTCTGAAC1464310.11629477543630634Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1460650.11600410004783197No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA1459840.11593977024874341Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTG1404910.11157725683647667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1365770.10846877740891211No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1357460.10780880132196625No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTC1351490.10733466687683185No Hit
ACGTGTCAACTATAAATAAATAAATAGAATTAGATCGGAAGAGCACACGT1332670.10583999179183531No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG1321490.10495208172540273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1320610.10488219256096079Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTC1315430.10447079952481404No Hit
ACATATACTAAAATTGGAACGATACAGAAGATCGGAAGAGCACACGTCTG1306740.10378064402595008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1291300.10255440686801455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)