FastQCFastQC Report
Sat 24 Jun 2023
ERR2812384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76675894
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA14220891.8546754733632451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA12006641.5658950125837463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT6448030.8409461779474002No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC5254900.6853392540816022No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA4460050.5816756437166549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3401430.44361139108466086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC2931700.38234963390188836No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG2892210.37719938420281085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2760170.3599788481109852No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2607820.3401095003861318Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC2466440.3216708500327365No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG2390790.3118046461903659No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1788600.23326757689972288No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC1787270.23309411951558073No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1668660.21762511174633323No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTC1665320.21718951199969053No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1621180.21143281355154464No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1579840.20604128854369796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTGA1536920.20044370138025389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1524620.1988395466246536No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1478980.192887219547776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC1385990.18075954875726655No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1359700.17733083099102828No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1304670.17015386869828997Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1263080.164729738918988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1235860.1611797314029361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC1213030.15820226367363907No Hit
TTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGA1204480.15708718048986817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1181610.1541044959971383No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1173360.15302853853911375No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT1172560.1529242032704568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTCAACTATAAATAAATAAATAGAATTAGATCGGAAGAGCACACGT1160020.1512887479342595No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC1148490.14978501587474155No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT1136400.14820824912716377No Hit
ACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC1134230.14792523971093186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1104040.14398788750999109No Hit
TAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGAA1048010.1366805061314316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC1015730.13247057804112464Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA987680.12881232268384116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT959140.12509016197450532No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA948090.12364903107618151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC913820.11917956900509045Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGAAC880560.11484183021067873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACG822590.10728143580562623No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA819710.10690582883846128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA815810.10639719440375876No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA801630.10454785176681473No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT776170.10122738184180806No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC771550.10062484566531432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)