FastQCFastQC Report
Thu 6 Jul 2023
ERR2812388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133782505
Total Bases11.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG104546147.814634656452276TruSeq Adapter, Index 4 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC60416464.516020984956142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT25993021.942931177735086No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC25420281.9001198998329416Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA23650801.7678544739463504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA19833971.482553342830589No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT13269830.9918957639491053No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC12270940.9172305452046963No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC10927160.8167854234752145No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA6942050.5189056670750783Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC5962550.44568981571992544No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC5893120.4405000489413769Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA5677230.42436266236754944No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC5515700.4122885873605073No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG5313960.3972088876643474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG5252210.3925931869791196No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT5150010.38495392203935785No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG5117430.38251862603409914No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT5085160.3801065019675031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT5028020.37583539043464614No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC4810820.35960008373292157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT4139500.30942012933604435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT3985260.2978909686285214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG3851970.28792778248546025No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA3698550.27645991529310954No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC3688680.27572215066536543No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA3504400.26194755435323924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT3009830.22497934240355272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT2982940.2229693635950381No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC2888110.21588099280993428No Hit
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA2844510.2126219717593119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2836330.212010531571374No Hit
GTCGCGGCGTGGGAAATGTGGCGTAGATCGGAAGAGCACACGTCTGAACT2430270.18165828185082944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG2398570.17928876425209708TruSeq Adapter, Index 4 (100% over 50bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC2148960.1606308687372837No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2146800.16046941264853726TruSeq Adapter, Index 4 (100% over 50bp)
AAGATGCTGCTGCTGCTGTGTTTGGGACTAGATCGGAAGAGCACACGTCT2141130.1600455904155779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2121000.15854090936628823Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA2058560.15387363243048857No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT2051090.15331526345690716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGAAC2022410.15117148538966288Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTC2013320.15049202434952164No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC2006810.1500054136376053Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1992400.14892829223073673No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1917090.14329900609948962No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1891470.14138395749130275No Hit
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG1832390.13696783447133093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1830390.13681833809286198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA1804940.134915996676845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1797990.1343964967616655TruSeq Adapter, Index 4 (100% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1775860.13274231933390693No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG1724420.12889727247968633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTCT1713830.12810568915569343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1699370.12702483033936313No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1690850.12638797576708555Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA1683220.12581764708322662No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT1629760.12182160888675243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1618620.12098891405868055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1589200.11878982233140276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1562600.11680152049776613Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT1481630.11074915961545195No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1474540.11021919495377965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1471940.11002484966177006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1419950.1061386913034705No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1373700.10268158755137677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1343350.10041298000811093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)