FastQCFastQC Report
Thu 29 Jun 2023
ERR2812389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117725556
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA49447634.200246036637958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT33559962.850694542483197No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC19608411.6656035160284144No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT18884291.6040943565388641No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT14307601.2153350968246859No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT10871860.9234919221787324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA10718370.9104539714384531Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC8922940.7579441799365977No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG8822860.7494430521101128No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG8729580.7415195388841485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC8696410.7387019688401387No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA8282300.703526089101673No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA8065830.6851384078406901Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC8055420.6842541478419519No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6739530.5724780777421005No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA6664860.5661353597684432No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT6346090.5390579765025701Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT6112490.519215216108217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG5742950.48782526030286916No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC5637990.47890960905718716No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACAC5516660.46860343560407564No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT4762200.4045170956763202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG4753590.403785733660073No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA4695440.39884627939238615No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG4648260.39483865338465673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTGACAAAACAGAAAAGGCTGGTGAAGATCGGAAGAGCACACGTCT4464920.37926514443473935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC4241330.360272666709682No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTG4207700.3574160227368134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA4016930.3412113848925037No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTC3995580.3393978449335164No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC3844650.3265773491016683No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG3834790.32573980793091345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG3756750.31911083095670406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT3676380.31228393603849275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC3386950.28769878988721875No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3361240.2855148970373094No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3312450.28137051227857446No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT3302260.28050493981102964No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAAGATCGGAAGAGCACACGTCTG2916810.24776353572711096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA2858710.24282832862560447Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2827330.2401628071308493No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2683710.22796324699456083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA2664810.22635781817840808No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACA2561360.21757043135137116No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCA2477280.21042839670258173No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT2458230.2088102263878881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2448350.2079709863506612No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2424980.20598586087798984No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2282970.1939230594927069No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCAC2266210.19249940938907095No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT2264750.19237539213660626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTCTG2213270.18800250983737124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2209410.18767462860825224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2203340.18715902263396403No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT2183800.1854992300907035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC2154590.18301803560817329No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC2152840.18286938479186288No Hit
AATCGCTGCCTCCAGGCCCGAGAATGAAGAGATCGGAAGAGCACACGTCT2087120.17728690956447893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2036210.1729624449596993No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGT2014190.17109199297389602No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACACG1913540.16254244745295574No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA1884040.16003661940657984No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTGAA1833630.1557546264636032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCAC1766110.15001925325372853No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC1704340.14477230415458814No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTCT1696280.14408766096632408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1686360.14324502319615293No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1518310.12897029766417073No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC1513840.12859060100765207No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG1474410.12524128575786891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT1463570.12432050012998028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGA1453390.12345577709567156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT1424390.12099242071109861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1404020.11926212520924513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC1386890.11780704607587497No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1332900.11322095603438899No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1311740.11142355530688682No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA1296980.11016979185046279No Hit
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC1284670.10912413953687337No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA1279510.1086858319870666No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1273720.10819401014338806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT1261510.10715685216215924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC1253580.10648325160596396No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG1226250.10416175057181298No Hit
AAGATGCTGCTGCTGCTGTGTTTGGGACTAGATCGGAAGAGCACACGTCT1202040.10210527270731258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTCT1198480.10180287447527536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1197900.1017536073475839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)