FastQCFastQC Report
Thu 29 Jun 2023
ERR2812390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121034880
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA17834251.4734802066974413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA11994170.9909680581333249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG9165900.7572940957185235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA7330330.6056378128354405No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT6395440.5283964424139553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC6005430.4961734997382573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT5633150.4654154240496624No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT5328830.44027225870757253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG5140320.42469740954012597No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG4595090.37965006451032957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4510190.372635557617771No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT4338550.358454521539576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC4124590.34077697272059093No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4083080.3373473828370797No Hit
GTTGCTGACAAAACAGAAAAGGCTGGTGAAGATCGGAAGAGCACACGTCT3770590.31152920546539975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT3691080.30496002474658546No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA3380040.2792616475515157No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT3347670.27658721188470625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGAAGATCGGAAGAGCACACGTCTG3347060.2765368131897185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC3331680.27526610510953536No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA3133170.25886504782753533No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2928070.24191951939804462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC2794210.23085989757663247No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA2752970.22745261531221414No Hit
TTGCTGAGACTTGCTAAGAAATATGAAGCAGATCGGAAGAGCACACGTCT2503480.20683954906222077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG2469340.20401887455913534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT2379830.19662348572576765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT2366120.1954907544007149Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCA2206110.18227059835974557No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT2112940.1745728173564513No Hit
CATGTCTTGGAGAATTCCTTAGTTCTTAAGATCGGAAGAGCACACGTCTG2079130.17177940772114617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2048870.1692793019665075No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCAC2036700.16827380669109598No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2001620.16537546862524258No Hit
TTGCTGAGACTTGCTAAGAAATATGAAGAGATCGGAAGAGCACACGTCTG1979010.1635074120782373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1967320.16254157479232434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1962660.16215656181094243No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA1903990.1573091988028575No Hit
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT1866130.15418117488115823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC1837450.151811610008619No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1771450.1463586364525664No Hit
CATACTGTAAGAGATGAAGAGTGCTCCGAAGATCGGAAGAGCACACGTCT1740320.1437866505919616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC1706160.14096432367264708No Hit
CTTAAGACAGACTATGATAATTATATTATAGATCGGAAGAGCACACGTCT1696430.1401604231771866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACAGACTATGATAATTATATTATGATAGATCGGAAGAGCACACGTCT1689330.13957381541585367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCTGTGACGTATGATGGATTCAATAAGATCGGAAGAGCACACGTCTG1659050.13707205724498592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTGCACACTTCCAGAGAAGGAGAAGCAGATCGGAAGAGCACACGTCTG1652490.13653006472183885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATACTGTAAGAGATGAAGAGTGCTCGGAAGATCGGAAGAGCACACGTCT1594570.13174466732234544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGC1526230.12609836106748734No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1467440.12124108356202773No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAG1465720.12109897576632456No Hit
GTAACAGACTTTGCAAAGACGTGTGTTGCAGATCGGAAGAGCACACGTCT1418930.11723314799832907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT1410340.1165234352279277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCGGAATAGATCGGAAGAGCACACGTCTG1379990.11401589360025804Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCT1362170.11254359074012382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATACTGTAAGAGATGAAGAGTGCTCCGAGATCGGAAGAGCACACGTCTG1354880.11194128502461438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1342950.11095561874395216No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1340490.11075237154777201No Hit
GTCAAAACCAACTGTGATCTTTACGAGAAAGATCGGAAGAGCACACGTCT1324610.10944035306186119Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1295310.10701956328621964No Hit
AAGAAAGTGATTAATGATTTTGTGGAGAAGATCGGAAGAGCACACGTCTG1284180.10609999365472167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT1270290.10495239058360697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCTG1247010.10302897809292659Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAAAACCAACTGTGATCTTTACGAGAAGATCGGAAGAGCACACGTCTG1236890.10219285548099853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1231460.10174422447479603No Hit
GCCTCTGACAAAAGAGAAAAGATAGAAGAGATCGGAAGAGCACACGTCTG1224070.10113365667814105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCTGACAAAAGAGAAAAGATAGAAGAAGATCGGAAGAGCACACGTCT1224010.10112869942945373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)