FastQCFastQC Report
Sat 24 Jun 2023
ERR2812395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87854090
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG31603753.59729979560428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA25443412.896098519716043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA16911641.9249690025814392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG13402061.525490731279557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT11811821.344481514747919No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA9952841.1328829426154206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA9207721.0480695890197032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8907231.0138662867033281No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA8745040.9954049948044537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA8467020.9637593423368224No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT5777810.65765976290916Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC5747300.6541869593094641No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5486380.6244877159390075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA4885250.5560640375422476No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4735150.5389788910226034No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC4348970.4950219164526091No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3760840.42807796427007555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3294960.3750491297559396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT3249880.3699178945453763No Hit
AAAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACG3167740.3605683013733339No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT2794510.3180853617628957No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC2699830.3073084019195919No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2532660.2882802610555752Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2429430.27653009666368406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2337940.26611623886833274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2228960.2537115801893799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG2193320.24965485386053168No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1895170.21571790226271764No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1845030.21001071207954006No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1837910.20920027741451763No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1792080.2039836733839028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC1780780.20269744982845989No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG1763010.2006747779187059No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1694870.19291873605429183No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1678980.19111005532013364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1656720.18857630874100456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1652740.1881232848692645Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA1629830.18551555197942407No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAG1629280.18545294817805294No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT1526870.17379612036275147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1513490.17227314061303234No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT1494490.17011046383839384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1489150.16950263783962705No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1479540.16840877869203358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT1438410.16372715260040824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC1393010.15855949335995628No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1385140.15766368987488233No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG1379360.15700578083501862No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT1348300.15347037343395167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1319080.15014440420474448No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT1274630.14508487880302443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1240380.14118636935400503No Hit
ACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC1228660.1398523392593333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1160030.13204052309915223No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1154280.13138602881209058Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT1120550.12754670841164026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1112930.12667936119991682No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCTG1089370.12399764199936508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTCT1056140.12021523414561576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1023370.11648518583483136No Hit
ACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGAACTC1012010.11519213277378436Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG997200.11350638314050034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG975380.11102271960246814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT974980.11097718956510734No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG953420.10852312055135965No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT951810.10833986215098237No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC919200.10462802585514232No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA911430.10374360487940858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG909510.10352506070007668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCA907090.10324960397404379No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT903680.10286146040554286No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTG879150.10006933086439118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)