FastQCFastQC Report
Wed 19 Jul 2023
ERR2812396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176248222
Total Bases9.8 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG79047044.484983683977249Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA51546882.9246751777161193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA33361781.892886045681641Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG28418941.6124383938466058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG26438401.5000661964124666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC22019891.2493680645470568No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA21566521.2236446844836824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA19460711.1041648976180878No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT19044801.080566929066666No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT19029221.0796829485179147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT18513371.0504145681537713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC15938770.9043364987818147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA15760240.89420703489423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA12807900.7266966925771313No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT11752970.6668419043682607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC9366760.5314527371515838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA8921590.5061946100086048Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA8014590.4547330979599896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT7489590.42494556342247813No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA6885480.3906694729663713No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA6850920.38870860212138764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6376640.36179882711100486No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC6295200.357178071277224Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT5942880.3371880823853077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG5596950.31756065034233366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA5371450.3047661950314597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG5079410.2881963824860599No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT4816210.27326289850458746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG4798090.2722348030268356No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC4502710.2554754850236163No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT4490610.25478895327522794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4411760.2503151492784988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA4258260.24160584156134068No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG4210480.23889489222762203No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4039700.22920514908797207No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC3917490.22227117842924965No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3761450.21341775578309097No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG3552730.20157536681419688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3481710.19754582261828435No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC3374860.19148335011288795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG3356080.1904178074488604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3306310.1875939491747043No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3210390.1821516247692984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG2981740.16917844425119932No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT2937990.16669614970640667No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2933960.16646749491748064Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG2756370.1563913649012584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2600310.14753680749187925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2587940.14683495644001449No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC2491040.1413370286368052No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC2393650.13581129913469425No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2349030.13327964238981088No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC2341510.13285297141891167No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC2332730.13235481036512245No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2041140.11581052999218341No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT2013660.11425136532724853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1981020.11239943175143066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1924380.10918578231104085No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1908390.10827853911626979Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1795270.10186031834125396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)