FastQCFastQC Report
Fri 23 Jun 2023
ERR2812400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99962696
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG26984492.699456005068131TruSeq Adapter, Index 8 (100% over 49bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA11836621.1841037180509817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTC6872480.6875044666662452No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5006230.5008098220960348Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4963260.496511218544966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT4445420.4447078938327154No Hit
GCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTCT4293310.4294912174037403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCAACCTTAAGTTTAACACAAAGCAGATCGGAAGAGCACACGTCTGA4233480.4235059846725222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA4020040.40215401953544755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC3886620.38880704057841736No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA3624320.3625672520877188Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGTCCCTGGCCGGCCGTGCCGGAGATCGGAAGAGCACACGTC3313910.33151466823183723No Hit
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC3302060.33032922601447245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3216880.3218080472739551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC2726460.2727477458191004No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA2633980.26349629465775914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2499960.25008929330997637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTCC2266280.226712572858179Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG2088440.20892193623909464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT2020260.20210139190323556Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTTCAACCTTAAGTTTAACACAAAGCAGATCGGAAGAGCACACGTCTG1902440.19031499510577427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1672690.16733142131340675No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG1557570.15581512527433233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG1509200.15097632020648982No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1468260.14688079241080093No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG1449440.14499809008752623No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC1430060.1430593668662158No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1366610.13671199904412343No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC1328410.13289057349953828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGATAAAACTGCGTGGCGGGGGTGCAGATCGGAAGAGCACACGTCTG1279540.12800174977273523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1257980.1258449451983568No Hit
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC1245030.12454946193127886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACG1234570.12350307158582438No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC1225750.12262074244176048No Hit
ATCCGGTCAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAA1224130.12245868198672834Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTCAACCTTAAGTTTAACACAAAGCAGATCGGAAGAGCACACGTCTGAA1212400.12128524424751409Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1133420.11338429687810742Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC1105790.11062026578394805No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1097640.10980496164289127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT1087330.10877357689512496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1075960.1076361525903623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA1035270.10356563412415368No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG1033340.103372562100566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1008110.10084862056941722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)