FastQCFastQC Report
Fri 25 Aug 2023
ERR2812401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126206593
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC42015043.3290685534946656No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA25008601.9815605037369164No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG21317851.6891233249597348No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC18878301.495825182445104No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC17591431.3938598279093073No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA16738681.3262920424450408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA11956960.9474116776133874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA10090560.7995271689173956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT9874590.7824147507095767No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG9676620.7667285654403173No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG7787930.6170779049554092No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC6984980.5534560306211578No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC6301820.49932573649302137No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC5924880.4694588340563159No Hit
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACGT5416900.42920895582689567No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG5248280.4158483225991213No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT4827860.3825362752641615No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC4306800.34125000109938786No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACACG3814630.30225283080100257No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACACG3773550.29899785029455633No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCACA3700290.2931930822346183No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC3468530.27482954079902944No Hit
TGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT3123830.24751718002561088No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2803290.22211914079639247No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA2682680.21256258775640985No Hit
GGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCA2530000.20046496303089334No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACGT2450260.19414675111307378No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2388000.18921356984892224No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2325700.18427721917824055No Hit
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA2037740.16146066156781524No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2019480.16001382748680967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCAC1988250.15753931333840854No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGTC1948400.15438179208276387No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACA1921440.1522456120814544No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1879010.14888366410461615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1809830.14340217551075163No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1808160.1432698527881186No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1795100.14223504155603028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAAAGATCGGAAGAGCACAC1786070.14151954803185282No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1775890.14071293406993404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1748900.13857437701372702No Hit
CTCAATCAATGAAATTGACCTACCCGTGAGATCGGAAGAGCACACGTCTG1686030.13359286229999093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACAC1661110.13161832203171825No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTTGACACAAGATCGGAAGAGCACA1610850.12763596272660652No Hit
GTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACACGTCTGAACT1609180.1275036400039735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1587170.12575967406076796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACTTCCGGGAGATCGGAAGAGCACACGTCTGAAC1573350.1246646441046071Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1572120.12456718485380554No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCACA1549480.12277330075775043No Hit
GGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1547860.12264493979328006No Hit
AAAGCATATCTTAGACTTAATAGTTAATCAAAAGATCGGAAGAGCACACG1524160.12076706642417644No Hit
GCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACGTC1477140.11704142904800544No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1472620.11668328611010045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAATCAATGAAATTGACCTACCCGTGCAGATCGGAAGAGCACACGTCT1445960.114570876657767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACG1417100.11228414984627624No Hit
CGAACTTAATGATAGCTGGTTATCCAAAATAAGATCGGAAGAGCACACGT1359270.1077019803553369No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1354590.10733115979131137No Hit
GCCAATTATAGCTCTAACAATCTTCAAATAAAAGATCGGAAGAGCACACG1347760.10678998362629122No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCAC1314500.10415462209648588No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA1279560.10138614549241495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1272400.10081882172352122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACG1272350.10081485996535854No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1269990.10062786498008071No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1265000.10023248151544667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)