FastQCFastQC Report
Fri 22 Sept 2023
ERR2812402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159614499
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA40055322.509503851526671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC34160442.1401840192475245No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA28491391.785012651012362No Hit
GTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACACGTCTGAACT20281091.2706295560279897Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG18894991.1837890741993307No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA18380611.15156267852584Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA16288781.0205075417365437Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC14330950.8978476322505013No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC11894800.7452205203488438No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC10096740.6325703531481811Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC8520770.5338343354384115No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT8402910.5264502944685495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8390220.5256552539127413No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG6479990.4059775296478549No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG6159640.3859072978075757No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG6120310.3834432359431207Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA5914400.3705427788236205No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5627470.352566341733153Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG5460410.3420998740220962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA5336770.3343537105610938No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC4694360.29410611375599405No Hit
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA4632820.29025057429149964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC4618400.2893471475921495No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC4469540.2800209271715347Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTG4428890.2774741660530476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGATAAAACTGCGTGGCGGGGGTGCAGATCGGAAGAGCACACGTCTG4248370.266164416554664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC4222490.26454300996803554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACGT4085600.25596672141921145No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3968040.24860147573435668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC3776040.23657249332969432No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCACA3718130.23294437681378807No Hit
AGCCCAGCCCTTGGGAAGGTCTGAGACGAGGGCCAGATCGGAAGAGCACA3654140.2289353425217342No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3215420.20144911772708068No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA3214810.20141090064756587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC3016750.18900225348575633No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG2984880.18700556770848242No Hit
GTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGAACT2943670.18442372205798171Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2919690.1829213522763994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2903450.18190390084800503No Hit
CCGCGGCGGTGGCGGGCGGGGGGGGACAGATCGGAAGAGCACACGTCTGA2736250.1714286620039449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2659880.1666440089505904No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA2637390.16523498908454426No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT2637320.16523060351804256No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2625710.16450322598826064No Hit
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA2589870.16225781593939032No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC2527750.15836593892388184No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2525740.15824001051433303Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGATAAAACTGCGTGGCGGGGGTAGATCGGAAGAGCACACGTCTGAA2519700.15786159877618636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG2466420.15452355615889257No Hit
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTC2444860.153172801676369No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACACG2419170.1515632987702452No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2404180.15062416102938117No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT2376350.14888058509020535No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCAC2314350.1449962261886998No Hit
GACCTGGTATTGCAGTACTTCCGGGAGATCGGAAGAGCACACGTCTGAAC2298180.14398316032680714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2240150.14034752569689798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGGCGGTGGCGGGCGGGGGGGGAAGATCGGAAGAGCACACGTCTGAA2218160.13896983130586402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC2167450.13579280163013263No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT2163750.13556099311504277No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCAGATCGGAAGAGCAC2143940.13431987779506172No Hit
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC2101730.13167538119453673No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCAC2063650.129289633017612No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2051670.1285390746363211No Hit
CCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGA2047570.12828220574122154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA1965660.12315046642473251No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1825930.11439624917783942Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGACCCCACACCCCCGCCGCCGCAGATCGGAAGAGCACACGTCTGA1769780.11087839833397592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1715460.10747519872865685No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCAC1685970.10562762221244072No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1681260.10533253623782636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCGGGGTTTCGTGCGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1677030.10506752271922365No Hit
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAAAGATCGGAAGAGCACAC1642240.10288789616787883No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG1633120.10231651950365736No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT1631940.10224259138262871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)