FastQCFastQC Report
Fri 23 Jun 2023
ERR2812406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102073266
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC21109252.0680488464041114No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG18622201.8243954298474196TruSeq Adapter, Index 4 (100% over 49bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA14412851.41201027113211No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA5872560.575327921808635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG5703240.5587398369324246No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT5075680.4972585084129668Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4739710.46434391547733955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC4379510.42905553742152225No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC4192570.41074124149216507No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT4009180.3927747349634135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG3979460.3898631008828502No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3855580.377726720334392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3838690.37607202653827104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCCGGAGCGCAGCGCATGTTCAGATCGGAAGAGCACACGTCTGAACTC3555440.34832235112375065Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG3249740.3183732751335692No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3105690.3042608629765996No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2808510.2751464815478717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC2765530.2709357805794124No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG2357890.23099976050536092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2262220.22162708108115206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC2149970.21063007820284696Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGGGCGCGGGCCATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2102830.20601182683818503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACTTCCGGGAGATCGGAAGAGCACACGTCTGAAC2000310.19596806082407514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1848730.18111794326244052No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1807800.17710807842672538No Hit
CTAAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1800390.17638212928348937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT1754430.17187948115621185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGAGATCGGAAGAGCACACG1725580.1690530799710083No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1669230.1635325355416765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1605510.15728996072291837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1598370.15659046316789746Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1580620.15485151616486925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGAGCTGGTATCAGGCACACACTAGATCGGAAGAGCACACGTCTGA1568010.15361612902637994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1550120.151863466385018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1508370.14777326709620517Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCGGCGGTGGCGGGCGGGGGGGGAAGATCGGAAGAGCACACGTCTGAA1505810.14752246685238818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1505300.14747250274131524No Hit
GATAAGAGGAGCTGGTATCAGGCACACACTAGATCGGAAGAGCACACGTC1480830.14507520509826735No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1470010.14401518219275947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGGCGGTCCCTGGCCGGCCGTGCCGGAGATCGGAAGAGCACACGTC1420220.1391373133882088No Hit
AGAGGAGCTGGTATCAGGCACACACTAGATCGGAAGAGCACACGTCTGAA1402430.137394447631371Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGCGGAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1384930.13567999283965304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGGGGCGCGGGCCATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1331610.13045629401140157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACGT1321960.12951089465482568No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1310890.12842637953800753No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACACG1302410.12759560373036366No Hit
GATTCAACCCGGCGGTCCCTGGCCGGCAGATCGGAAGAGCACACGTCTGA1257450.12319092444832715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1234820.12097388948052275No Hit
TGGGCAGGTGGCTCGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAG1216220.11915166895903968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1166300.1142610642046077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1157030.11335289300922338Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1120250.1097495988812585No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1106230.10837607567097932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC1083670.10616589852234179No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA1061450.1039890307810862No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCACA1031060.10101175757421144No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1028440.10075507919967996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)