FastQCFastQC Report
Fri 23 Jun 2023
ERR2812407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82517690
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC22342892.707648505429563No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA18909692.2915922634286057No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA16532442.0035025217017104No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC6313710.7651341185144664No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6258750.7584737284817352No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC5980860.7247973131579423No Hit
GGTGGGGCGCGGGCCATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA5437330.6589290126759486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG5188340.6287548766815939No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG4005080.48536016943760785No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC3214330.3895322324219207No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2699990.32720135524879573No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA2523420.30580351922115123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2499920.3029556450259332Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA2466860.2989492313708733No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2406460.29162958875848316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC2395890.29034865130131493No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2241740.2716678084420444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA2058950.24951619464868685No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2037290.24689130294364758No Hit
CTAAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1914980.23206902665355755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1844500.22352782779062283No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1840060.2229897613469306No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1813050.21971652381446938Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1801730.21834469675532606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC1794440.21746124982412862No Hit
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTC1776760.21531867894023696No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1764830.21387292833815388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1640630.19882161025108674No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1618370.1961240068644675No Hit
GGTGGGGCGCGGGCCATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG1576420.1910402484606634No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1563140.18943089657502532Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1521240.18435319747801956No Hit
GGTGGGGCGCGGGCCATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1490480.1806255119356831No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC1442940.17486432303182506No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACA1394060.16894074470577156No Hit
AAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACT1365040.16542392304001724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT1358130.16458652684048716No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1338000.162147049923477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC1308780.15860599103052933No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1262800.15303385249877935No Hit
AACCCGGCGGTCCCTGGCCGGCCGTGCCGGAGATCGGAAGAGCACACGTC1194890.14480410200527913No Hit
ATTCAACCCGGCGGTCCCTGGCCGGCCGTGCCGGAGATCGGAAGAGCACA1168930.14165810991558295No Hit
CACCCGGAGCGCAGCGCATGTTCGTGGGGCAGATCGGAAGAGCACACGTC1052940.1276017300048026No Hit
GTGGGGCGCGGGCCATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGT1003080.12155938926550175No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC999640.1211425089577762No Hit
GGCCGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAG939540.11385922218617608No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT926830.11231894639803901No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAAGAGATCGGAAGAGCACACGTC917280.1111616187995568No Hit
TGGGGCGCGGGCCATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTC916340.11104770383174808No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC895170.10848219333333253Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGAGATCGGAAGAGCACACG886500.10743150953450102No Hit
GTGGGGCGCGGGCCATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC878770.10649474070347825No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA874720.10600393685281301No Hit
CGGTGGGGCGCGGGCCATGTGGCGTACGGAAGAGATCGGAAGAGCACACG855080.10362384114242654No Hit
GTGGGGCGCGGGCCATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA832290.1008620091037449Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGGGCGCGGGCCATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA830970.10070204340426883Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)