FastQCFastQC Report
Mon 26 Jun 2023
ERR2812408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157234827
Total Bases15.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA39672522.523138210340639Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGCGATCTATTGAAAGTCAGCCCTTGACACAGATCGGAAGAGCACAC36862862.3444462466321156No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA33870112.154109916119283No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTTGACACAAGATCGGAAGAGCACA32740132.0822441582868914No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC31428021.9987950888259634No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT29975541.9064186078825909No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC27241411.7325302873262296No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC24203051.5392931999728026No Hit
CTGGGTCGGGGTTTCGTGCGTAGCAGAGCAGCTCAGATCGGAAGAGCACA23070311.4672519085100655No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC22103931.4057909702155236No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG20449891.3005954463256413No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACA19616061.247564574227566No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA19021321.2097396208538456No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT16226971.032021360000606No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG12409470.7892316375938773No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT11337790.7210737097068196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACACG9389770.5971813102195228No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACG8154680.5186306466314871No Hit
AGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACT7308470.46481241716251576Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCTGAAC6575480.41819488248618103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC6546660.4163619552301857No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCAC6252790.3976720755383284No Hit
TCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC6213000.3951414657008527No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5836730.3712110167552129No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACAC5610980.3568535105775262No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5418730.34462657563772436No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG5035550.32025665662480746No Hit
GCTGCGATCTATTGAAAGTCAGCCCTTGACACAAGATCGGAAGAGCACAC4710840.29960537941126747No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC4367950.27779786980654103No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAAGATCGGAAGAGCACAC4057920.2580802279891846No Hit
GGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCA3980730.2531710102622493No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAAGATCGGAAGAGCACA3786780.24083595678201752No Hit
GCTGCGATCTATTGAAAGTCAGCCCTTGACACAGATCGGAAGAGCACACG3512510.22339262026217638No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG3322410.21130242347644776No Hit
ATAAGAGGAGCTGGTATCAGGCACACACTAGATCGGAAGAGCACACGTCT2945280.1873172792691787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGTC2679460.170411355494416No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGTC2608040.16586910481352837No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTC2606140.1657482664448125No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2586500.16449917930713912No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2561260.1628939369774611No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACGT2542150.1616785573847453No Hit
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2520600.16030799588694175No Hit
TAAGAGGAGCTGGTATCAGGCACACACTAGATCGGAAGAGCACACGTCTG2513840.15987806569087903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTGCGTAGCAGAGCAGCTAGATCGGAAGAGCACAC2480850.15777993001512317No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCACA2423070.15410517162333254No Hit
CACCCGGAGCGCAGCGCATGTTCGTGGGGCAGATCGGAAGAGCACACGTC2234720.14212627333510533No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1945520.12373340163372329No Hit
AGGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGC1895820.1205725243046822No Hit
TGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1894590.12049429736072402No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTTGACAGATCGGAAGAGCACACGT1870040.1189329384386323No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG1850420.11768512328378751Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC1803590.11470677549064877No Hit
CTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACACGT1684500.10713275373782172No Hit
GGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA1636910.10410607059719663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC1604150.10202256272396953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACA1586250.10088413809238332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)