FastQCFastQC Report
Sat 24 Jun 2023
ERR2812412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372136872
Total Bases18.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG83960692.2561776678769956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA45606211.2255224738923478No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA45304181.2174063740719572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA40699631.0936736739164077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTCGATGGTACTTGCCGTGCCTACCAGATCGGAAGAGCACACGTCT34774800.9344626296531026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGAATCCCCGCCCCGCGGTGGGGCGCGGGCCAGATCGGAAGAGCA28098950.7550703011229696No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA28057170.7539475959264794No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA27018600.7260393159858666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT24278030.6523951757191102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG23342170.6272469017797301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG22180210.5960229063246386No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT18877940.5072848572769214No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT18737030.5034983472425167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC16670990.4479800647112442No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC15067300.4048859743196852No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA14887790.40006221151877686No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC14757240.3965540936776617No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC14223650.38221555213158237No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC14209400.38183262850664257No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC13971590.37544223782264713No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT13079890.3514806240430806No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG12539040.3369469929870319No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA12241940.3289633713049536Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC11916450.3202168582746619No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC11880970.3192634456281451No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC11792170.31687722682852026No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT11790360.31682858881019454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG11738310.3154299098854144No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC11678120.3138124942373353No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC11308870.3038900697805618No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG11153500.2997149930362182TruSeq Adapter, Index 4 (100% over 49bp)
AGAATCCCCCCTAAGCGTGACGATACCAGATCGGAAGAGCACACGTCTGA11060240.2972089258599454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG10869580.2920855421174175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTAGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCT10838790.29125815836921426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG9904660.26615637270149356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC9776870.2627224211203667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA9389650.25231710981866906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCAGATCGGAAGAGCACA9205210.2473608688794482No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT8870940.2383784211525269No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA8634540.23202591975352554No Hit
ATTTCCACCGAGGCGGTGCGCCGCGACCGTCTCAGATCGGAAGAGCACAC7778010.20900938835214372No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGA7653950.20567566870933443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTC7642450.2053666426260497No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC7630590.2050479426827665No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTC7533970.20245158614650796No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG7520180.20208102356489951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCAGATCGGAAGAGCACAC7345690.19739215736730326No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCAGATCGGAAGAG7272350.19542137711094643No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA7103800.19089212960332508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGT6604680.17747986015210016No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC6521810.1752529913241169No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA6475980.17402145520264384No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCAGATCGGAAGA6379550.17143020431471784No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT6206730.16678621407878122No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTC6060350.16285271511606622No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT6058100.1627922534910757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6045210.16244587555946352No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGT5986090.16085721277304657No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG5882870.15808350213681593No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCAGATCGGAAGAGC5690220.15290664344596308No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCA5616530.15092645804794103No Hit
AGAAAAGGTGTTGGTTGATATAGACAGCAGATCGGAAGAGCACACGTCTG5432700.14598660892705093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG5428190.14586541695873662No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT5407560.1453110510371571No Hit
ATGTGGCGTACGGAAGACCCACTCCCCGGTGCAGATCGGAAGAGCACACG5290140.14215576036765312No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC5277340.1418118009010405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAACGATGCCGACTAGCGATCCGGCGGCGTTAAGATCGGAAGAGCACA5259590.14133482585944884No Hit
AGACCCCAGAAAAGGTGTTGGTTGATATAGACAGATCGGAAGAGCACACG5259340.14132810790111655No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGC5164700.13878495759484968No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT5104410.1371648547634377No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA5081100.13653847232853616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGC4988990.13406330776059192No Hit
TACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAA4867010.1307854815311072Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGCGCGAGTCAGGGGCTCGCACGAAAGCCAGATCGGAAGAGCACACG4855620.13047941134948865No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA4820810.12954400283130235No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG4697040.12621807602015853No Hit
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG4658360.12517867350698858No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC4636340.12458695573708159No Hit
CTCCTCGGGGGCTAGGCCGCGACGAGTAGGAGGGCAGATCGGAAGAGCAC4588660.12330570672394968No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCAC4548410.12222411543245304No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCAGATCGGAAGAGCAC4542770.12207255829247686No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCT4539560.12198629970749042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC4445700.11946410943122024No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGT4421780.11882133517798796No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA4396110.11813153521642972No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG4338860.11659312275833823No Hit
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGCCGGGCAGATCGGAAGAG4097820.11011593605268978No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC4093620.11000307435270751No Hit
ATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGA4061360.10913618900951046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT4045510.10871027045124407Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGGGCGCGACCCGCTCCGGGGACAGTGTCAGATCGGAAGAGCACACG4026120.10818922560299266No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGATCGGAAGAGCACACGTC4001130.10751769848809822No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCT3975340.10682467390654049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGATCGGAAGAGCACACGTC3958110.10636167221827995No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC3955230.10628428133829211No Hit
AGCCCAGCCCTTGGGAAGGTCTGAGACGAGGGCCAGATCGGAAGAGCACA3929490.10559260034840084No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAG3866290.10389430048200114No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAG3800420.10212425282061274No Hit
ATCCCGGGGTCGAGGAAGATGACCGCCGCCGCGCAGATCGGAAGAGCACA3790480.10185714679732139No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGT3776530.10148228472238031No Hit
ACGATCCTTCTGACCTTTGGGGTTTTAAGCAGATCGGAAGAGCACACGTC3731760.1002792327442361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)