FastQCFastQC Report
Fri 23 Jun 2023
ERR2812413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96320471
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC77090928.003586278144342No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA42299054.391491191939873No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC12844361.3335026154512886No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC12514991.2993073922987772No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG11569071.2011018924523325No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC5762740.5982881873573894No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC5147840.5344492138125031No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC4957680.5147067854350504No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG4746790.49281216658502425No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT4104810.42616174499395876No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC3968500.41201002848086155No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3896640.40454951678963447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA3862570.4010123663120376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA3827520.39737347214591595No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACA3799440.39445820400940523No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC3782840.39273479050990107No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3505990.3639921985016041Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3300520.34266028454117503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2923670.30353568349972043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2816680.29242797203514503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCAC2730810.2835129408783726No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT2553880.26514405229600674No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG2541740.2638836763993814No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2520090.26163597144370276No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT2429770.25225894088495476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2065100.21439886854373874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGCATATCTTAGACTTAATAGTTAATCAAAAGATCGGAAGAGCACACG2059110.2137769862026526No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2052590.21310007921369073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2044330.21224252526755188No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1953000.202760636417569No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCACA1925350.19989001091990094No Hit
CTGGGTCGGGGTTTCGTGCGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1877650.19493779261108474No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1668490.17322278251733217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1634620.16970639605780166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1579410.16397448887059532No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1569630.1629591283871525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1553230.16125647890571465No Hit
TGGGCAGGTGGCTCGGGGGTGGCGGGGGAGATCGGAAGAGCACACGTCTG1531690.15902019415997248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACACG1504080.15615372146591766No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1447120.15024012912063106No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1436130.14909914632788704No Hit
AGCCCAGCCCTTGGGAAGGTCTGAGACGAGGGCCAGATCGGAAGAGCACA1424560.1478979478827507No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1418160.14723349930462862No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACG1383260.1436101781520566No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1369200.1421504676819946No Hit
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA1293410.13428194303576443No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1275500.13242252521792588No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1256450.13044475249710935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1229390.1276353808527369No Hit
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACGT1159900.12042092277559564No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1134620.11779635089201339Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1093610.11353868898751544No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1080580.11218591321049501Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGAGATCGGAAGAGCACACG1075120.11161905551728458No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1054840.10951358408536022No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1029030.10683398755390223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCCTGGATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC986120.10237906747777427No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACGT982030.10195444330831814No Hit
AGAGTCAGTAACTAATTTTTAGAGGCCTCAGATCGGAAGAGCACACGTCT977030.10143534285666025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC970990.10080826951105752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)