FastQCFastQC Report
Mon 26 Jun 2023
ERR2812414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135932990
Total Bases13.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACAC63766414.691017978784988No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACA58448714.299817873497816No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA46573353.426199188291231Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC44346163.2623544880459114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC38930192.8639250854409957No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCAC28735172.113921719812093No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA24680361.8156269497198583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA23165421.704179390153928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACAC21163711.5569222747178593No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACG19669741.4470173870228265No Hit
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCA10338550.7605622446765866No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA9597980.7060817245320654No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCACA8303160.6108274378427194No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAGATCGGAAGAGCACACGTC7150320.5260180034294839No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA6876050.5058411501137435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACA6560850.4826532543718784No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6285400.4623895935784242No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC5588030.41108710990613834No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCAC5314000.390927912348577No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACAC5050980.37157867269748135No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4627640.3404353865827567No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4427970.3257465314343486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4420990.3252330431339736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCACACG4393530.32321293013564995No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGAAGATCGGAAGAGCACACGT4373580.32174529523701345No Hit
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACGT4263960.3136810276887163No Hit
GGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCTAGATCGGAAGAGCAC3947920.29043133679322436No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT3933170.2893462433218014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCAGCCCTTGGGAAGGTCTGAGACGAGGGCCAGATCGGAAGAGCACA3766660.2770968254284703No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3738690.2750391939440161Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3648160.268379294827547No Hit
GTCGCCTGGATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC3595820.2645288682313248No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3377690.24848199101630886No Hit
GTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG3328440.24485888230664243No Hit
GGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACACGT3121230.2296153420887748No Hit
GACCTGGTATTGCAGTACTTCCGGGAGATCGGAAGAGCACACGTCTGAAC2945630.2166972123544108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGGGCGCGGGCCATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2902710.21353977426671775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2740590.2016133096167457No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2723540.20035901512943988No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC2674500.19675135520818016No Hit
GGCCGGGGCCCTCCGTCCCCTGAAGTGCAGACGGAGATCGGAAGAGCACA2566390.18879817180509306No Hit
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTC2556390.18806251521429787No Hit
GGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGCA2554580.18792936137136393No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC2481210.18253184896469946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC2474800.18206029308999971No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAAGATCGGAAGAGCACAC2342690.17234153386900414No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG2184590.16071080316853179No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2163780.15917990180308694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTTGACACAAGATCGGAAGAGCACA2061930.1516872394258377No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2034920.14970023097409982No Hit
CCGGGGCCCTCCGTCCCCTGAAGTGCAGACGGAGATCGGAAGAGCACACG2024550.14893735508944517No Hit
TGCAAGATTTTATTACTCTAGAGAAGAGTTAAAAGATCGGAAGAGCACAC2002550.1473189105896957No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA1968170.14478972323054176No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCAATGGGAGATCGGAAGAG1933050.14220609728366895No Hit
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGACAGATCGGAAGAGCACA1914020.14080614279138567No Hit
AAAGCATATCTTAGACTTAATAGTTAATCAAAAGATCGGAAGAGCACACG1871370.13766856743164407No Hit
GGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAAGATCGGAAGAGCACA1837730.13519381866020896No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1803890.13270435675695796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1802500.13260210049083745No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1800090.13242480725245578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGAGATCGGAAGAGCACACG1765300.12986545797307925No Hit
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGAGATCGGAAGAGCACA1758930.12939684472474267No Hit
GCCAATTATAGCTCTAACAATCTTCAAATAAAAGATCGGAAGAGCACACG1754270.1290540287534321No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1734130.12757241637957056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCTCGCCGCATCCCGGGGTCGAGGAAGATGAGATCGGAAGAGCACACG1726580.12701699565352015No Hit
AGCGCCGAGGAGCCTCGGTGGGCCACGGATAGCCGGGCAGATCGGAAGAG1705990.12550227873307282No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1639830.12063517472837167No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1629440.11987082753053543No Hit
GCCGCGACCGTCTCCGGGACGGCTGGGAAGGCCGGGAGATCGGAAGAGCA1612590.1186312461750455No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1601030.11778082715608623No Hit
CGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGCAGATCGGAAGAGCA1573220.11573496617708476No Hit
AGCTCTCGCCGCATCCCGGGGTCGAGGAAGATAGATCGGAAGAGCACACG1559560.1147300592740585No Hit
GCGCCGAGGAGCCTCGGTGGGCCACGGATAGATCGGAAGAGCACACGTCT1551350.11412608521301562Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCGGTGCGGAGGGCCGTTCGCGCCGGGGGAGATCGGAAGAGCAC1516100.11153289573046249No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1496530.11009321578227624No Hit
CGGTGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1489510.10957678485553801No Hit
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT1483630.10914421878015043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGGTGCGCCGCGACCGTCTCCGGGACGGCTGGGAAGATCGGAAGAGC1432220.10536220824687223No Hit
ATGCAAGATTTTATTACTCTAGAGAAGAGTTAAGATCGGAAGAGCACACG1428770.10510840672304789No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1397690.10282198603885635No Hit
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC1371880.1009232563780139Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCAATTATAGCTCTAACAATCTTCAAATAAACAGATCGGAAGAGCACAC1364100.10035091555037523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)