FastQCFastQC Report
Sat 23 Sept 2023
ERR2812418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524355445
Total Bases33.5 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG304130475.800082232387231TruSeq Adapter, Index 1 (100% over 49bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT273011905.206618956726959Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT269965035.148511998383082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA168143603.20667214583802No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC106335942.0279362217741443No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA89125351.699712491781219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG47762330.9108769720127536No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC39665530.75646263194616No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC35694330.6807277456611517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT32537210.6205182059280419No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTG29869070.5696340199156319Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC28060140.5351358561748129No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC25504790.4864026919754786No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA24005750.4578144506537927Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT22172390.4228503815765659Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG21847600.4166563007656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACGTCTGAACTC20744010.39560969944729Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA20285840.3868719242535948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCAC19904640.3796020464705959No Hit
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC19500800.3719004005002751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCAC18272260.3484708736075011No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC17018760.3245653337308245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACA16922600.32273146319668716No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT15476470.295152270231503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTCCGAGAGTTGTGGGTTATTGTTACAGATCGGAAGAGCACACGT14594870.2783392475308424No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC14472180.2759994224909784No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC14082000.26855828683155947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC14047450.2678993826411014No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACAC13782360.26284384250076775No Hit
AGTTGGTCCGAGAGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC13666350.26063141196140344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT13363190.25484983759442037No Hit
CCCCACAACTGAGTTTGACTGGCTTTGCTTTAGATCGGAAGAGCACACGT12875390.24554698769267097No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA12625660.24078437861935428Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG12275750.2341112334592044No Hit
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA11839700.22579530951566643Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGA11477170.218881487918944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC11297100.21544736700502845No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTAGATCGGAAGAGCACACGTCTG10962620.2090684878842061Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC9451890.18025730618664595No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA9327400.17788315328736598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT8124910.1549504268044742No Hit
GGACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGC8069680.15389713365139174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC7411360.14134229120096195No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG7331860.13982614407675312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAGATCGGAAGAGCACACGTCT7035130.13416719645201738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC7030100.13407126915598255Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC6557600.12506020605926957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAAC6533960.1246093668389388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACACG6519670.1243368417772414No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATCGGAAGAGCACACGTCTGAA6438160.12278236187668462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACACG5898430.11248915323078222No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG5798870.11059044118441452Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACGTCTGAACTCC5730230.1092814054786825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT5729530.10926805575557626No Hit
CCGCGGCGGATGCCCCTCCCGCCGGGGCTTGGAAACAGATCGGAAGAGCA5729200.1092617623146833No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC5501050.10491070613369907No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA5456890.10406852931602532No Hit
CCCTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACA5390940.10281079468908728No Hit
GTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACACGTCTGAACT5361890.10225678118017828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACAC5354420.10211432056360166No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCAGATCGGAAGAGCACACGT5349620.10202277960515886No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCA5343350.10190320422819295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)