FastQCFastQC Report
Fri 8 Sept 2023
ERR2812419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226598576
Total Bases17.5 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10264470.45298034000001836No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA8676500.38290178840311867No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG5139270.22680063090952524No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC4835760.2134064602418331No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT4245840.18737275736454762No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC3019040.13323296435896403No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2945040.12996727746426792No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2598450.1146719474530149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)