FastQCFastQC Report
Sat 24 Jun 2023
ERR2812420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140295978
Total Bases14.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT28783252.051609063233445No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGTC25072561.7871189436378567No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACG23479861.6735946628491376No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC20885201.4886527965897924No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA16148951.1510629335361275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG15301061.090627131164088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAAGATCGGAAGAGCACACGT13798060.9834964762853002No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC11293290.8049617787332436No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC10069630.7177418870838905No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC8983100.6402963312319616Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG8021540.5717583721466342No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC7511800.5354251851753014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGGGCGCGGGACCTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA7213390.5141551527585487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6442600.4592148749980559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGTCT6405010.4565355394578739Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGAGATCGGAAGAGCACACGTC5999620.4276401993505473No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCAGATCGGAAGAGCACACGT5738620.40903667245542846No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC5429140.38697759389795194No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5209220.3713021623470917No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT5152840.3672835154262227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA5088270.36268110266140346No Hit
AGTTGGTCCGAGAGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC4232490.30168291780966094Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG4169600.2972002518846264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTAGATCGGAAGAGCACACGTCT4023020.28675234011341366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACAGATCGGAAGAGC3964500.28258115852758087No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG3958240.2821349589936213No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG3812880.27177400623701414No Hit
AGTAATAACTTACAAGTTTAAACGCAGATCGGAAGAGCACACGTCTGAAC3789070.27007687989458967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA3713440.2646861337678547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTAGATCGGAAGAGCACACGTCTG3711190.26452575853600024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC3646690.25992833522283865No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3624670.2583587962870896No Hit
AACGATGCCGACTCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG3451070.24598495617600669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3407990.2429143050700997No Hit
CCCCACAACTGAGTTTGACTGGCTTTGCTTTAGATCGGAAGAGCACACGT3177900.2265139774712572No Hit
ACGATGCCGACTCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA3123520.2226378863120367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA3107120.22146892906651966No Hit
GGTGGGGCGCGGGACCTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA3070820.21888154199260082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC2674080.1906027555544037No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACACG2642760.18837033232698944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2613330.18627262429433294No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGTC2585800.18431034423524245No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGT2504450.17851188863019293No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCAC2444500.17423877967478155No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2444250.1742209602045755Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC2390130.17036340129436925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGTCT2339440.16675032551539004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2322830.1655663999148999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCA2297630.16377019731812983No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2182420.15555827266837258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2182000.1555283359584264No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACGT2124990.15146478397263818No Hit
GTGGGGCGCGGGACCTGTGGCGTACAGATCGGAAGAGCACACGTCTGAAC2039530.14537337627740118Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTAATAACTTACAAGTTTAAACGCAGATCGGAAGAGCACACGTCTGAACT1962020.13984862773471668Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1929360.13752069214699797No Hit
GGGCAGGTGGCCCGTGGGGGTGGTGGAGATCGGAAGAGCACACGTCTGAA1917550.13667890037446404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1910330.13616427407491324No Hit
CAACTAGCCCTGAAAATGGATGGCGCTGAGATCGGAAGAGCACACGTCTG1908130.1360074627371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1886790.13448639276031135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAAGATCGGAAGAGCACACGTC1878440.1338912224554292No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1847500.13168588482272814Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1766300.1258981208998023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG1723460.12284457648529311No Hit
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT1713240.12211611654326969Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACGTCTGAACTCC1680640.11979245762840043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1670730.11908609382943253No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1645560.11729202956908716Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTGGTCCGAGAGTTGTGGGTTAAGATCGGAAGAGCACACGTCTGAACT1606920.11453785225403966Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACGTCTGAACTC1600280.11406456712536692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACAC1580420.1126489884121981No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTAGATCGGAAGAGCACACG1566370.11164753418661795No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1561550.11130397480104526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGT1551870.1106140049146669No Hit
AACTAGCCCTGAAAATGGATGGCGCTGAGATCGGAAGAGCACACGTCTGA1548530.11037593679271406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTAGATCGGAAGAGCACACGTC1544800.11011007029723974No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCTG1535240.1094286537565603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATAACAAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1476290.1052268226819731Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1473880.10505504298918678No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCAGATCGGAAGAGCACACGTCTGA1437970.10249545428878937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1428520.1018218783150006No Hit
GGTGGGGCGCGGGACCTGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC1423200.1014426799890158Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAACCATAAACTTATAAGTTATTCATAAGAGATCGGAAGAGCACACGTC1421190.10129941144855913No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC1412170.10065648496352475No Hit
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT1405290.10016609314345418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)