FastQCFastQC Report
Sat 24 Jun 2023
ERR2812424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205764142
Total Bases10.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC59075382.871024048495291No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA54294032.6386536289690357No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC47713022.318820934310313No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC40718801.9789065093761575No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG29622431.43963033170279No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA21231191.031821666964694No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC17028780.8275873451264409No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG16146060.7846877421431377No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC12677860.6161355363851492No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT10585560.5144511525239417No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACAGATCGGAAGAGC10416520.5062359213200519No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG10305310.500831189527668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACGT8966270.43575473903514245No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG7101710.3451383672088016No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC6772800.32915356068211343No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG6266330.30453945663671567TruSeq Adapter, Index 2 (100% over 49bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACAC6203850.301502970328037No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG5182260.25185437800916743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC5176820.25158999763914164No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGTC4983660.24220255052991693No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG4914020.23881809299892495No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACG4300410.20899705644533534No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4294220.2086962265757656No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA4282570.20813004434951546No Hit
GTCCACCCGGAGGATTCAACCCGGCGGCTCCGGAGATCGGAAGAGCACAC4061290.1973759840040545No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3865350.1878534307498534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC3837550.18650236930008923No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACA3823320.18581080079540777No Hit
GATCAGCTGAAGGGGATCGTGACCGACTAGATCGGAAGAGCACACGTCTG3315810.16114615344397568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCAAACAGGACTAGGATTGCTGAACAGATCGGAAGAGCACACGTCT3240670.1574943995829944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCTGACTTCGGCGATATCCATAAGGAGATCGGAAGAGCACACGTCTG3165780.15385479555519446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGCTGCTTACCAGAAGTTTGGAGAAAAGATCGGAAGAGCACACGTCTG2897460.14081462259833397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC2706200.1315195142212874No Hit
CGGGTGACCCAGTACGAGACGGAGATCCAGATCGGAAGAGCACACGTCTG2648190.128700266929891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCTGGATACTCCAGCTAGGAATGATGGAATAGATCGGAAGAGCACAC2631600.1278940039999778No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2559860.12440748787026265No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2483080.12067603110361182No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2480890.12056959856494336No Hit
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2464880.11979152324801082No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCAGATCGGAAGAGCACACG2456700.11939398070631763No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACAC2332670.11336620546839497No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA2313200.1124199764602328No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2272910.11046190934472927No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2206210.10722033385194976No Hit
CCTATTCAAGAGTTCATATCGACAATAGGGAGATCGGAAGAGCACACGTC2183920.10613705472550218No Hit
CCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGCACACGTCTG2157700.10486278022144402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA2141190.10406040523814883No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2139270.1039670945193162No Hit
GTGCGCCGCGACCGGCTCTGGGACGGCTGGGAGATCGGAAGAGCACACGT2138140.10391217727333658No Hit
CCCGGCTCTCGCCGCATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACAC2066140.10041302531711284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)