FastQCFastQC Report
Fri 23 Jun 2023
ERR2812425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95708059
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACG17726491.8521418347853025No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA17700611.8494377782752862No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC16976611.7737910660167082No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC13616651.4227276304913885No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACAGATCGGAAGAGC7201080.7524005893798348No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC7187710.7510036328288717No Hit
CTAAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA5410690.5653327479977418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4993640.5217575251421618No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC4924150.514496903547067No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA3718600.38853572403970704No Hit
GGTGGGGCGCGGGACCTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2842710.29701887486820727Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC2834820.2961944928796435No Hit
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2804530.2930296601250685No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGAGATCGGAAGAGCA2722070.28441387574268956No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACGT2602980.2719708274514271No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACAC2513740.2626466387746929No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG2468960.2579678269308544No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACA2456070.2566210228963059No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG2077500.21706636010662383No Hit
GGTGGGGCGCGGGACCTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2037000.21283474153414814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACA1969150.20574547437013638No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACGT1906910.1992423647417194No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1830970.19130781870730446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1824370.19061822160660472Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAAGATCGGAAGAGCACACGTC1811560.18927977632479204No Hit
AGCCTCAAACAGGACTAGGATTGCTGAACAGATCGGAAGAGCACACGTCT1705580.1782065186381013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACACG1643460.17171594713878796No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGT1636270.17096470423666202No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1612410.16847170623322325No Hit
CCCCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1603780.16757000578185374No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCA1493380.15603492700651259No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1401700.14645579637133796No Hit
CGGTGGGGCGCGGGACCTGTGGCGTACAGATCGGAAGAGCACACGTCTGA1368670.14300467633556335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG1364980.14261912886562667No Hit
AAGGCTGACTTCGGCGATATCCATAAGGAGATCGGAAGAGCACACGTCTG1357070.14179265718887896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1334060.13938847093325757No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACCAGATCGGAAGAG1281390.1338852771008552No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGTC1259230.13156990259305124No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACAC1244640.130045475062868No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTAGATCGGAAGAGCACACGT1207930.12620985240124868No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACAC1203290.12572504474257493No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGTCT1186370.12395716853896284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACT1161690.12137849331998259Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1126930.11774661525629727No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1078360.11267180750160234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTGCACTAAAGCGGACGCTGAGAAAGAGATCGGAAGAGCACACGTCTG1046320.10932412703093268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACG1042970.10897410426012295No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1034910.10813195992199569Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCACCCGGAGGATTCAACCCGGCGGCTCCGGCAGATCGGAAGAGCACA1023300.10691889593121932No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCAC1013740.10592002497929669No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAAGATCGGAAGAGCACACG998640.10434231040042302No Hit
TGCGCCGCGACCGGCTCTGGGACGGCTGGGAGATCGGAAGAGCACACGTC958750.10017442731755745No Hit
GCTGACACCTTCGGGGAGAAGACCAAGGCAGATCGGAAGAGCACACGTCT958140.10011069182794732Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)