FastQCFastQC Report
Fri 23 Jun 2023
ERR2812426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109091022
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA41231763.7795740881408193No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC31806332.9155772323775646No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC28986962.657135249865016No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC23833462.1847315721361564No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC16645871.5258698373913848No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACAGATCGGAAGAGC14447991.3243977125817008No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA12073131.10670243789631No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC9625430.8823301701216072No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT9118810.8358900515204634No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG8642290.7922090967302515No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACG8362720.7665818732544278No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC8150260.7471063934115495No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA8116060.7439713966562711No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC7943220.7281277463877825No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG6749810.6187319429457724No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACGT5727570.5250267065973587No Hit
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA5262230.48237058407977884No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACAC3944150.3615467091324894No Hit
GCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACG3708250.33992256484681205No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGAGATCGGAAGAGCA3707700.3398721482323266No Hit
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC3557180.3260744958462301No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC3152760.2890027008822046No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACCAGATCGGAAGAG3148410.2886039512949104No Hit
AGCCTCAAACAGGACTAGGATTGCTGAACAGATCGGAAGAGCACACGTCT3057910.28030812654775566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2958390.2711854693230392Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC2801690.2568213175232697No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2544520.23324742525558154No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACACG2331090.21368302883806514No Hit
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC2166230.1985708778124748No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG2070750.18981855353779709No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2001770.18349539341560114No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2001180.183441310138244No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1990120.18242747785422708No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA1983170.18179039518027432No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1925760.1765278172937091No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1875190.17189223875819953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1855240.17006349065095383No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGCAGATCGGAAGAGC1843240.16896349178945266No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACA1836330.16833007577837159No Hit
GTCCACCCGGAGGATTCAACCCGGCGGCTCCGGAGATCGGAAGAGCACAC1694870.1553629225327085No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCA1668520.1529475083659955No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGAGATCGGAAGAGC1659990.15216559250861175No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1536310.14082827090940628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1517600.13911318935118236No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1468560.1346178606705142No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCACA1428450.13094111447594653No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA1419330.1301051153412056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1378660.12637703586643453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCAGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1320610.12105579137392258No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAAGATCGGAAGAGCACACGTC1318590.12087062489890323No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACCGAGATCGGAAGA1276670.11702796220939245No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1276090.11697479559775323No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG1269900.11640737951836219No Hit
GCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACA1247270.11433296499871455No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1224650.11225946714478485No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACAC1222050.11202113405812625No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACA1187350.108840304016952No Hit
CCCGGCCTCTCGCCGCATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACA1168940.10715272243026562No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1107820.10155006156235295No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC1095500.10042072939787841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)