FastQCFastQC Report
Sat 5 Aug 2023
ERR2812430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182171001
Total Bases18.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCAC40593782.2283338059936333No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT40240312.2089306080060456No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG29529711.6209885128753285No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC25378941.3931383074521286No Hit
CCCCACAACTGAGTTTGACTGGCTTTGCTTTAGATCGGAAGAGCACACGT21144641.1607028497362213No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA20419451.1208946477710797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC18562011.018933304318836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA17072150.9371497058414912Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA15972620.8767926789840716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG12919280.7091842241125963No Hit
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12694420.6968408764466305No Hit
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC11568060.6350110575502629No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG10901990.5984481580578239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGGTCCGAGAGTTGTGGGTTATTGTTACAGATCGGAAGAGCACACGT10293720.5650581016459365No Hit
CCCCACAACTGAGTTTGACTGGCTTTGCTTTTAGATCGGAAGAGCACACG10264810.5634711311708717No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTC9651050.5297797095598108No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC9336910.5125354720974499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCA8799790.4830510867094593No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGC8743060.4799369796513332No Hit
GCGGGCAGGTGGCCCGTGGGGGTGGTGGTGGTGGTGGAGATCGGAAGAGC8201120.45018800769503375No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCA8041750.44143963396237806No Hit
CGACTGCATAATTTTTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG7979440.4380192212919772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7581630.4161820464498628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCAC7526930.4131793731539083No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACAGATCGGAAGAGCACA7292860.4003304565472526No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT6937980.38084985875441285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA6882280.3777922919795561No Hit
GCCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG6515180.3576408958745306No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG6510560.35738728800200203No Hit
GTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACACGTCTGAACT6331760.34757233397427506Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCTGGTGTAGTGGTATCATGCAGATCGGAAGAGCACACGTCTGAAC6078650.33367824552932Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCAGATCGGAAGAGCACACGT6062310.33278128608405677No Hit
CCCCGCGGACGGGAGGGCGGTGGTGGTGGTGGGGGAGATCGGAAGAGCAC5890010.3233231396691947No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCT5748790.31557108257861527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCGCCACGCTTCCTTCGGCTCCTCTCCCCAGATCGGAAGAGCACACGT5239900.28763634010003597No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5057470.2776221227438938No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC4808080.2639322380404552No Hit
GGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCTGAAC4713550.25874315747982307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGGCTTCAGTCGCCACGCTTCCTTCGGCTAGATCGGAAGAGCACAC4637440.2545652148005708No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA4564030.2505354845143547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4551730.24986029472385674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGTC4444400.24396857763327545No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4092190.22463454542910483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCA4001920.2196793110885964No Hit
GGACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGC3975230.21821420413669462No Hit
AGTTGGTCCGAGAGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC3970440.21795126437275272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCGCCACGCTTCCTTCGGCTCCTCTCCCAGATCGGAAGAGCACACGTC3964590.21763013752117438No Hit
TCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACACGTCTGAACTC3932080.21584555052206142Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT3912860.2147904978575597No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT3825050.20997030147515083No Hit
GCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCTGA3735830.20507270528748978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT3689120.20250863088796442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTAGATCGGAAGAGCACACGTCT3575450.1962688891411427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT3560840.19546689541438048Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3526360.19357416826183No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC3515450.19297528040700618Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA3431400.18836148350526988No Hit
GGGGGCCCGGTGCGGAAGGCCCGTCCGTCTCGGAGATCGGAAGAGCACAC3347730.18376854612551644No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT3338120.18324101979326554No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACA3258360.17886271591602004No Hit
GATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCTGAACT3221700.1768503209794626Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3209330.17617128864544143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGATCGGAAGAGCACACGT3204630.17591328929460073No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCACA3147790.17279314395379536No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCAGATCGGAAGAGCACACGTCTG3145720.17267951445246765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACA3143500.1725576509293046No Hit
GCTTGACTGAACTGGGCAATGAAGCACGCACACAGATCGGAAGAGCACAC3118150.1711661012391319No Hit
CGGGCAGGTGGCCCGTGGGGGTGGTGGTGGTGGTGGAGATCGGAAGAGCA3071970.16863112038342482No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA3036260.16667087425182453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAGATCGGAAGAGCACACGTCT3033730.16653199375020175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2970940.16308523220992785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG2962640.16262961633503895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGCCACGCTTCCTTCGGCTCCTCTCCCCCAGATCGGAAGAGCACACG2922050.1604014900263956No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACA2858600.15691849879004616No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG2784570.15285473454691068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACAC2745230.15069522508689515No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC2715510.1490637908939195No Hit
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGAGATCGGAAGAGCACA2711510.1488442169783104No Hit
CAGTGCGCCCCGACCGCGTCGCGCCGCTGGGTTAGATCGGAAGAGCACAC2688580.1475855095070812No Hit
GGACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAG2675840.14688616658586623No Hit
CCCCCACAACTGAGTTTGACTGGCTTTGCTTTAGATCGGAAGAGCACACG2650000.1454677190910314No Hit
GACTGCATAATTTTTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA2612830.1434273284802338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGGCCCGGTGCGGAAGGCCCGTCCGTCTCGGAGATCGGAAGAGCACA2577380.14148135465314812No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2573670.14127769984642066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC2504810.13749773488920997No Hit
GTCGCCACGCTTCCTTCGGCTCCTCTCCCCAGATCGGAAGAGCACACGTC2494750.13694550649145304No Hit
GTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACGTCTGAACTC2475370.13588167087032693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGGTCAGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA2474540.13583610928283807No Hit
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2466840.13541342949529053No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTAGATCGGAAGAGCACACGTC2456840.13486449470626777No Hit
CGGTGGGGCGCGGGACCTGTGGCGTACGGAAGAAGATCGGAAGAGCACAC2423420.1330299546413537No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC2420770.13288448692226265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGGGGCGCGGGACCTGTGGCGTACGGAAGAAGATCGGAAGAGCACACG2367640.12996799638818474No Hit
AGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACT2345760.12876692706980294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC2331390.12797810777797725No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTTAGATCGGAAGAG2323270.12753237272929077No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTAGATCGGAAGAGCACACGTCTG2287400.1255633436410661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC2238750.1228927758924704No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2171400.11919570008840212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2061780.11317827693113462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGTC2039300.11194427152541143No Hit
GGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACAC2035250.11172195293585722No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGAAGATCGGAAGAGCACAC2033090.11160338302142832No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACGT1992280.10936318014742642No Hit
TCGCCACGCTTCCTTCGGCTCCTCTCCCCCAGATCGGAAGAGCACACGTC1981230.10875660720555627No Hit
CGGTGGGGCGCGGGACCTGTGGCGTACGGAAGAGATCGGAAGAGCACACG1963600.10778883517250915No Hit
GGTGGGGCGCGGGACCTGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1950330.10706039870747594No Hit
GGGCAGGTGGCCCGTGGGGGTGGTGGTGGTGGTGGAGATCGGAAGAGCAC1936990.10632811969891959No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC1841830.10110445624657902No Hit
CGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCTG1834380.10069549982875704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)