FastQCFastQC Report
Fri 23 Jun 2023
ERR2812431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98001941
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA35469333.619247704491894No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG26065002.659641200371736No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC16843431.7186833064867562No Hit
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC16223451.6554212941557962No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA12499731.275457391195956No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC12402061.265491262055718No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGC11196021.1424283933315158No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCA9696710.9894406070998124No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA9597540.9793214197665737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG9556140.9750970136397604No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9408620.9600442505521396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCAC8910800.9092472974591391No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7394220.7544973012320236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC7000060.7142776896633098No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC6710600.6847415399660298No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC6256020.6383567443832566No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT5804940.5923290845841512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC5703870.5820160235397787No Hit
GCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACG5220320.5326751640561894No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC4964670.5065889460291404No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4481110.45724706615759786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT4055900.4138591499937741No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGCA2868470.29269522325073133No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTAGATCGGAAGAGCACACGTCTG2807640.28648820333058506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2805710.2862912684555911Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCA2746890.28028934651406545No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2707680.2762884053490328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2639310.2693120129120708No Hit
AGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGCAC2628850.26824468711288074No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2397310.24461862444132612No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2363190.24113706074454178Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2281460.23279743000192207No Hit
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCA2267720.23139541695403765No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT2261170.23072706284460223No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCAC2230140.22756079902539889No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACA2195340.2240098489477877No Hit
ATTAACCATAAACTTATAAGTTATTCATAAGAGATCGGAAGAGCACACGT2173200.22175071001910052No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCAC2138640.2182242492523694No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT2135440.21789772510730168No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTACAGATCGGAAGAGCACACGT2053650.209551972036962No Hit
TCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACACGT2020870.20620714032592474No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1997170.20378882087651712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCTTCCGCGGGTGTTACAGCCCCCCGGCAGATCGGAAGAGCACACG1933380.1972797661221832No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCACA1920360.19595122100693904No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAGATCGGAAGAGCACACGTCT1879450.19177681388983916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1866580.19046357459389504No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG1858210.18960950987695233No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACAC1786150.1822565942852091No Hit
TGGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCA1739510.17749750487084742No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1682100.17163945763074223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1656110.1689874693400205No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC1579230.16114272675476907No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1573070.16051416777551375No Hit
TCCTCGCCGCCGTCGCCGTCCCCCGCCTCGGGGAGATCGGAAGAGCACAC1566770.15987132336491172No Hit
TCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACACG1517780.1548724427815159No Hit
AGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCACA1502320.1532949230056576No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCA1471830.15018376013593446No Hit
GCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACAC1470480.150046007762234No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACA1438030.1467348488536569No Hit
AGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCACAC1406830.14355123843924683No Hit
GGACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGC1387640.14159311395679397No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1377530.14056150173597073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1349890.13774114943294846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1340930.13682688182675892No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC1222340.12472610108814068No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCAC1195710.12200880796840545No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1150370.11738236898797749No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1085730.11078658125761No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGAGATCGGAAGAGCA1084770.11068862401408969No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1000440.1020836924036025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)