FastQCFastQC Report
Mon 26 Jun 2023
ERR2812432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143544551
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA24427421.701730914188446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA23473771.6352950938555655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCAC20592951.4346033936181946No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA16554481.1532642573106102No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC16531481.151661967301009No Hit
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGC16057171.1186192640638792No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA15409451.0734959908021864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACA14478911.0086701236050402No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA13042420.9085973594358173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG12084240.8418459576358284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCA10436680.7270690477132775No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG10105100.7039695989574694No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC9378980.653384606706527No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACAC9340610.6507115689818139No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC8746130.6092972487684329No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT8372840.5832920819126042No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG8163180.5686861635033432No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC7847740.546711104345577Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCAC7752170.5400532410317685No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6214930.4329617499726618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC6040880.42083659448696176No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA6004400.41829522320216805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5926230.41284952711301454No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG5783690.40291950894046824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCACA5503670.3834119763974879No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC5285830.3682362000630731No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC4848130.33774392453253066No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC4650830.32399906284147284No Hit
CCCCGCGGACGGGAGGGCGGTGGTGGTGGTGGGGGAGATCGGAAGAGCAC4647230.3237482696225787No Hit
CGACTGCATAATTTTTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG4413560.3074696997728601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT4314870.3005944823360101Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC4142780.2886058698250413Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4048760.2820559869249234No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCACA3991120.27804050883129655No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3882790.2704937228860746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTTAGATCGGAAGAG3757620.26177378199469237No Hit
CGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCAC3526760.2456909701852772No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCTGGCACTACAGATCGGAAGAGC3411020.23762796819783147No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCA3289470.2291602138210039No Hit
CTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACACG3285120.22885717201484018No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCA3268900.22772720923415618No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT3260140.22711694573484714No Hit
GGACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGC3153370.21967883685114595No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTTAGATCGGAAGAGCA3142180.21889928792908345No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3134830.21838725177384127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGCAC3079790.2145529021160824No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC2812910.1959607648220656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACA2716620.18925274286447838No Hit
GACTGCATAATTTTTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA2702590.18827534595862158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2684040.18698306423348665No Hit
GGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACA2683450.18694196201150123No Hit
CCCTCTCTCTTTCCCGCCTCTTTCCCCGTCCCAGATCGGAAGAGCACACG2507230.17466563394663445No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2415190.16825368731690832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACGT2311190.16100854988218952No Hit
CCCTCTCTCTTTCCCGCCTCTTTCCCCGTCCCCAGATCGGAAGAGCACAC2247200.1565507004163467No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTTACAGATCGGAAGAGCACACGT2241460.15615082456177665No Hit
CTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACACG2233550.15559977612803988No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACA2216380.154403631803481No Hit
CTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGCAGATCGGAAGAGCACAC2171120.1512506037237178No Hit
GAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGTAGATCGGAAGAGCAC2109940.14698851229817841No Hit
AGTTGGTCCGAGAGTTGTGGGTTATTGTAGATCGGAAGAGCACACGTCTG2090600.14564119539445283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGGGGGCCGGGCCGCCCCGCCCCCGGCGCGACGAGATCGGAAGAGCA2047280.14262331699376035No Hit
CGACTGCATAATTTTTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2040170.1421280003864445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC2005610.139720385485061No Hit
GCTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGACAGATCGGAAGAGCAC1987560.13846293615143915No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1978000.13779694082570923Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCATAATTTTTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1937060.13494486460861896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCCGCATCCTGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCACAC1924400.134062908455508No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1898980.13229202967098347No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCGAGATCGGAAGAGC1846040.1286039760575795No Hit
CCCTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACA1828930.1274120116200022No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1819270.12673904981596967No Hit
CCTCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACAC1768170.12317917940333381No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAGATCGGAAGAGCACACG1767000.12309767160719322No Hit
CCCCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1737760.12106067335150882No Hit
TCCGCCTTCCGCGGGTGTTACAGCCCCCCGGAGATCGGAAGAGCACACGT1720860.11988333851836702No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1699870.11842107472264829Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACACG1606840.1119401599577263No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG1582930.11027447499557125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCCGGCCGTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCA1546860.10776166627181828No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGACCAGATCGGAAGAGC1541540.1073910496261192No Hit
GGGCAGGTGGCCCGTGGGGGTGGTGGTGGTAGATCGGAAGAGCACACGTC1516740.1056633630070709No Hit
GCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAGATCGGAAGAGCACAC1506810.10497159171162129No Hit
AGCTCTCGCCGCATCCCGGGGCCGAGGGAGTCAGAAGATCGGAAGAGCAC1476380.10285169236413577No Hit
GTCGCCGGGCGCATTTCCTCCGTAGATCGGAAGAGCACACGTCTGAACTC1475420.10278481417243068Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1460520.10174680890534117Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCCGGGCGCATTTCCTCCGTTAGATCGGAAGAGCACACGTCTGAACT1440810.1003737160318959Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)