FastQCFastQC Report
Thu 22 Jun 2023
ERR2812436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204787235
Total Bases20.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTC65519373.1993874032236436No Hit
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGT40850521.9947786296348011No Hit
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTC22836321.1151241921890298No Hit
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTCT21539171.0517828418358206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG14632490.7145215862697691Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCTGAGGTGGGATCCCGGGGCGAGATCGGAAGAGCACACGTCTGAACT13617680.6649672280598934Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA13402010.6544358099273131Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC12059340.5888716647793013No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGCCAGATCGGAAGAGCACACGTCT10243240.5001893794796342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC9853150.4811408289193415Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTGAACTC9801710.4786289536064101Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9660250.4717212964958485No Hit
GGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTCT9508160.4642945640630384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA9331630.45567439786957425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA9327270.4554614939744657Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG9222510.45034594075162937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA7539310.36815331775928317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTG6960260.3398776295797929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCAGATCGGAAGAGCACACG6534090.31906725045630896No Hit
GCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAACT6425120.31374611801365454Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCTGAGGTGGGATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTC6384490.31176210763332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5886560.28744760385089435No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC5738850.2802347519365648No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGT5670630.2769034896144772No Hit
GCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTCTG5560870.2715437805486265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA5023170.24528726119086475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTGGGCTCGCGATAGCCGGCCGCCGCCCAGATCGGAAGAGCACACGT4651080.22711767166542388No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA4607580.22499351583119914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCATTTATCAGACCAAAACCAACAGATCGGAAGAGCACACGTCTGAAC4496560.2195722794929088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT4318600.21088228472834256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGCCGGCGGGCAGGTGGCCCGGCGCAGATCGGAAGAGCACACGTC4311480.21053460680789016No Hit
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT4213920.20577063799899442No Hit
AAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACT4202060.2051915003393644Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAA3978610.1942801757150537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC3967620.19374352117210822Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA3927170.19176830040212223No Hit
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC3903800.19062711599187324No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG3639180.1777054121561825No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG3608240.1761945757996098No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGT3602180.1758986589178764No Hit
TCCCCCGCCGCGTACCGCATGTTCAGATCGGAAGAGCACACGTCTGAACT3550400.17337018100761992Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACG3545340.17312309529448944No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT3425510.16727165636080785No Hit
CTCGGTGGGCTCGCGATAGCCGGCCGCCAGATCGGAAGAGCACACGTCTG3398080.1659322174060312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACT3266420.15950310574777768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT3223990.15743119926395802No Hit
CCCCCGCCGCGTACCGCATGTTCAGATCGGAAGAGCACACGTCTGAACTC3162820.15444419668052065Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCCACATTAGGAATTGGTGCTTAAGAAGATCGGAAGAGCACACGTCT3099930.15137320448708633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGGCTGAGGTGGGATCCCGGGGCAGATCGGAAGAGCACACGTCTGAA2999710.14647934476970695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAAC2965500.14480883049180288Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGAAGCAGCAGTATTTTTGTGGAATAGATCGGAAGAGCACACGTCT2943950.1437565188084111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGCATTCAGCTTACACCTGAAAGATAGATCGGAAGAGCACACGTCTGA2894070.14132082011850006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACAC2869750.1401332460980783No Hit
GGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTCTG2845320.13894030064911028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGGTTTCTATCTATGAACACACTCAGATCGGAAGAGCACACGTCTGAA2842450.13880015519522004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC2796110.1365373188421632Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCT2729710.1332949292469328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTGGGCTCGCGATAGCCGGCCGCAGATCGGAAGAGCACACGTCTG2715230.13258785392556327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTG2686180.13116930847765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA2677690.13075473185621164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2595190.1267261604464751Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2541470.12410295006912905No Hit
GCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGTCTGAAC2414540.11790480983836713Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCATTTATCAGACCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA2383480.11638811374156206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGA2377210.11608194231442208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2339530.11424198388146606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG2273450.11101522026018859Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAAC2241690.10946434234536152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCAC2239640.10936423844972565No Hit
GGCTGAGGTGGGATCCCGGGGCGAGATCGGAAGAGCACACGTCTGAACTC2219690.10839005663609844Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAAC2203560.10760240988653418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGTCTGAA2150200.10499677872988519Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGGTTTCTATCTATGAACACACTCCAGATCGGAAGAGCACACGTCTGA2126540.10384143328074134Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCTGCAGCCATGAAGTGGGTAACATTAGATCGGAAGAGCACACGTCTG2087540.10193701770522953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCTTGAAACTGGAAAGAAAATGACAACAGATCGGAAGAGCACACGTCT2048430.1000272307011714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)