FastQCFastQC Report
Sat 24 Jun 2023
ERR2812437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109804856
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGT38740713.528141779084889No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC22545482.0532315984276686No Hit
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTC21576351.9649722959429043No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACG15345751.3975474818709293No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA13698121.2474967409455917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC10013070.9118968290436992No Hit
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG9277810.8449362203070508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA7944840.7235417712309554No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA7614220.693431991750893No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCA7540900.6867546914318616No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGT7285800.663522567708663No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA7231910.6586147701883056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC6589400.6001009645693629Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCTGAGGTGGGATCCCGGGGCGAGATCGGAAGAGCACACGTCTGAACT5838880.5317506176593866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5668210.516207589216273Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA5420930.49368763800391485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCACACGTCTGAACT5017920.4569852539126321Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTGCCGGCGGGCAGGTGGCCCGGCGCAGATCGGAAGAGCACACGTC4840160.44079653453577683No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA4633230.4219512841945715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTC4441380.4044793793090535No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA4321640.39357457925175915No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG4254290.3874409707344819Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC4090660.37253907969243183No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACAC4089290.3724143128970544No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4065250.370224974385468No Hit
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA3873210.35273576607577356Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCC3787450.34492554682645366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT3533580.3218054400071341No Hit
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3306100.30108868773526737No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA3301170.3006397094132157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT3196650.29112100470310714No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG3011310.27424196977226584No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG2804180.25537850530034845No Hit
TGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC2774950.2527165100967848Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC2762270.2515617342096419No Hit
AGGGCTGCCGGCGGGCAGGTGGCCCGGCGCCAGATCGGAAGAGCACACGT2458810.22392543368027368No Hit
AGCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCAC2364090.21529922137505467No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2309770.21035226347366642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTCT2237700.2037888014715852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACT2105410.19174106471211075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2085190.18989961609712416No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGA2020410.18400005915949655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGT1911550.1740861078129368No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1861750.1695507892656405No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG1853720.16881949191755236No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGT1804080.16429874467482566No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACG1801280.1640437468448572No Hit
GCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAACT1800080.163934462060585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCT1752640.1596140702556907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1718100.15646848988172252No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1645890.1498922779881429Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1573210.14327326288738998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAAC1543340.1405529824655478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACG1543120.14053294692176455No Hit
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1537950.1400621116428585No Hit
CGGCTGAGGTGGGATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTC1505180.13707772632569185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGCTCCTGCTGAAGAACGAGAAGTAGGAGATCGGAAGAGCACACGTCT1498810.13649760626251356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1480020.134786388682118No Hit
AGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACACGTCTGAACTC1449640.13201966222696015Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAAGCAGCAGTATTTTTGTGGAATAGATCGGAAGAGCACACGTCT1414470.1288167073412491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACTCC1359790.12383696400457918Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1342080.12222410273002862Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCCACATTAGGAATTGGTGCTTAAGAAGATCGGAAGAGCACACGTCT1299950.1183872960955388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTGAGCATTGTGATTCATGATACGTGAGATCGGAAGAGCACACGTCTG1283390.11687916607258243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTCT1217040.11083662820886536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCCCAGATCGGAAGAGCAC1206340.10986217221577159No Hit
TTTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1180090.1074715675598172Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGAGATCGGAAGAGCACACGT1144710.1042494878368585No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGCAGATCGGAAGAGC1130670.10297085586087376No Hit
AAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAAC1118970.10190532921421983Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1109070.10100372974397416Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)