FastQCFastQC Report
Wed 27 Sept 2023
ERR2976734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14287455
Total Bases409.4 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCG146273410.237890513040986No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA9189886.432132244685985No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG8404895.882706192250475No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT6878234.814174392850231No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG5067133.5465588518039075No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG4699833.2894801768404522No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG3072202.150277988627086No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA2453651.717345741421408No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG2408551.685779587757232No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1975961.3830034810258371No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA1728101.2095226196687934No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1715831.2009346661109344No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG1205120.8434812218131221No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1171600.8200200805531845No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1135630.794844148240537No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1116630.7815457686480903No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1089650.762662069626816No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT977770.6843556112687669No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG976950.6837816812021456No Hit
CTCTAGCATCACCAGTATTAGAGG835960.5851007054790374No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC829360.5804812683574506No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT785420.5497270157631293No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT785070.549482045612742No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT712240.4985072568907479No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA705390.49371284109031316No Hit
ATTGATCATCGACACTTCGAACGCA603260.42223055120733544No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG598200.4186889827474522No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA576360.4034028453632925No Hit
CTCTAGCATCACCAGTATTAGAGGC528780.3701009032049445No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG505650.35391187583792916No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG469660.32872194523097364No Hit
TTGATCATCGACACTTCGAACGCA463690.3245434543800838No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC452040.3163894479457678No Hit
AACGATGCCGACCGGCGATGCGGCGGCG413750.28958971349341084No Hit
GAGGGCGCACCACCGGCCCGTCTCGC404640.2832134904361904No Hit
TTGATCATCGACACTTCGAACGCAC398150.2786710439332967No Hit
CGCCACGCAGTTTTATCCGGTAAAGC397940.27852406184306444No Hit
ACGATGCCGACCGGCGATGCGGCGGCG390510.2733236955077024No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA372840.26095620248672696No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG364870.2553778822050533No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA341540.2390488718949596No Hit
CCTCTAGCATCACCAGTATTAGAGGC327280.2290680880534707No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT316780.22171898354185543No Hit
TCTAGCATCACCAGTATTAGAGG296970.20785367302994132No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT285710.1999726333346282No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT285530.19984664868585764No Hit
AAGGGACAAGCATCAAGCACGCAGC282260.19755792756652601No Hit
ATTGATCATCGACACTTCGAACGCAC278310.1947932644407279No Hit
ACTCGCCGAATCCCGGGGCCGAGGG276080.1932324546254039No Hit
AGGGACAAGCATCAAGCACGCAGC270030.18899797059728274No Hit
GCCGCGACCGGCTCCGGGACGGCTGGG268150.1876821309323459No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC255010.17848525157209594No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG254280.17797431382985984No Hit
GAGGGCGCACCACCGGCCCGTCTCG252330.17660948013484556No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAG228530.15995150990851764No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG226720.15868466427365827No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG219000.15328132267083255No Hit
ACGATTAAAGTCCTACGTGATCTGAGT217770.1524204275709005No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT216130.15127256743765774No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT213830.14966276359225628No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG199330.13951400021907331No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG192280.13457960147556022No Hit
TTGATCATCGACACTTCGAACGC184250.128959286310963No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG183230.12824537330126323No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT182680.12786042020779767No Hit
GCACGAGCGCACGTGTTAGGACCCGAAA175970.12316399246751783No Hit
TTGATCATCGACACTTCGAACG174080.12184115365542708No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA169840.1188735152621653No Hit
TGCATAATTTGTGGTAGTGGGG169560.11867753914185557No Hit
ATTGATCATCGACACTTCGAACG169340.11852355790446935No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGA163650.11454104317388926No Hit
TGCTAAGACTTCACCAGTCAAAGCGA163360.1143380679064256No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCG162480.11372214295688071No Hit
ATTGATCATCGACACTTCGAACGC155480.1088227399491372No Hit
GGGACAAGCATCAAGCACGCAGC150990.10568012287702744No Hit
GGCGGGGCGCGGGACATGTGGCGTA150790.10554013993394903No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGA147530.10325841796177135No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG147490.10323042137315568No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT146530.10255850324637943No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG146330.10241852030330105No Hit
TGCATAATTTGTGGTAGTGGG145550.10187258682529533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)