FastQCFastQC Report
Wed 27 Sept 2023
ERR2976735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520862
Total Bases154.4 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1217372.2050360976238856No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1046651.895809024025596No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG1040021.8838000297779587No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA1026491.8592929872183004No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT933781.6913663119998292No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG890481.6129365305635242No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG829481.502446538239862No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA692171.2537353768306472No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG685511.2416720432425228No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT636721.1532981624970884No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG473380.8574385666586124No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC470100.8514974654320285No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA455920.825813070495151No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG441450.7996033952668985No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG431700.7819431096086081No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA399380.7234015267905628No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT363440.6583029968870803No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG356290.6453521207376675No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC341000.6176571702027691No Hit
TCGCTTCTGGCGCCAAGCGCCCGG339700.6153024654483303No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA331500.6004497123818708No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA328940.5958127553269761No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA314380.5694400620772626No Hit
CCGTCTCCGCCCCCCGGCCC304060.5507473289497183No Hit
GGCCGCCGGTGAAATACCACTAC284660.5156078887680946No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC272190.4930208362389786No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT264660.47938166177672975No Hit
AACGATGCCGACCGGCGATGCGGCGGCG243540.44112676607384865No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA238890.43270416829835634No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC236790.428900414464263No Hit
CTAAACCATTCGTAGACGACCTGCTT230020.41663783662768605No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG225900.4091752338674649No Hit
CCCGTCTCCGCCCCCCGGCCC224710.4070197733614787No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA219700.39794510350014184No Hit
ACGATGCCGACCGGCGATGCGGCGGCG208030.3768071000506805No Hit
GCGCAGCGGAAGCGTGCTGGGC201570.36510602873246967No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATG190050.34423972198544356No Hit
GAGGGCGCACCACCGGCCCGTCTCGC187320.3392948420011223No Hit
CGCTTCTGGCGCCAAGCGCCCGG184880.3348752423081758No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA183820.33295525227763345No Hit
GTCTAGGGGTATGATTCTCGCTT176140.31904438111294936No Hit
CGCCGCTGGTGTAGTGGTATCATGCA168190.3046444558838819No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG168100.30448143786242077No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG161160.2919109370964172No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGA160730.29113207321610285No Hit
CGGCCGAGGTGGGATCCCGAGGC159900.2896286847959612No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT157430.28515474576252764No Hit
ACTGACCCGGTGAGGCGGGG154920.2806083542751114No Hit
CGCCACGCAGTTTTATCCGGTAAAGC154190.27928609698992657No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC149610.27099029100890404No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC143630.26015864913848596No Hit
GGGGGGGATGCGTGCATTTATC143030.259071862328745No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA141930.25707941984421995No Hit
GCCGGGCGCGGTGGCGCGTGCCTGT135410.2452696698450351No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG133760.2422810061182475No Hit
CGCGCTACACTGACTGGCTCAGCG132490.23998064070429584No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC130830.23697386386401253No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG129740.23499953449298316No Hit
CGCTTCTGGCGCCAAGCGCCCGGC120280.21786452912606766No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA119060.21565472927959436No Hit
GACCGGCTCCGGGACGGCTGGG118480.2146041686968448No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT114480.20735892329857186No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC114120.2067068512127273No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA110170.19955217138193276No Hit
CATCTGTGGGATTATGACTGAAC109980.19920802222551476No Hit
AAACCATTCGTAGACGACCTGCTT108520.19656350765514516No Hit
CGCAGCGGAAGCGTGCTGGGC108190.19596577490978762No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC106700.19326692099893097No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT104080.18852128526306217No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG100800.18258018403647835No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG94440.17106024385322435No Hit
CGAGGGCGCACCACCGGCCCGTCTCG94160.17055307667534525No Hit
GGGGGGATGCGTGCATTTATC92620.1677636571970102No Hit
GTCTAGCGGTTAGGATTCCTGG91360.1654814048965542No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG90530.16397801647641258No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTC90450.1638331115684471No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC89900.1628368903261846No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT89720.16251085428326228No Hit
GGCCGAGGTGGGATCCCGAGGC89220.16160519860847816No Hit
AAGGGACAAGCATCAAGCACGCAGC88830.16089878718214656No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG87580.15863464799518626No Hit
ACCAGGAGTGGAGCCTGCGGCTT86790.15720371202902736No Hit
CGCGCGCTACACTGACTGGCTCAGCG86610.15687767598610505No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG85850.1555010793604332No Hit
GCGCCGCTGGTGTAGTGGTATC84760.15352674998940383No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG83350.1509728009865126No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC81820.14820149462167323No Hit
AGGGACAAGCATCAAGCACGCAGC81740.14805658971370775No Hit
GGCGGGGCGCGGGACATGTGGCGTAC81640.14787545857875092No Hit
ATTGATCATCGACACTTCGAACGCA81320.14729583894688908No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT81320.14729583894688908No Hit
CACTCGCCGAATCCCGGGGCCGAGGG79150.143365293318326No Hit
ACCGCAGCTAGGAATAATGGAAT78000.14128228526632253No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA77150.13974267061918955No Hit
ATTGATCATCGACACTTCGAACGC76640.13881890183090975No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT76260.1381306035180738No Hit
GCCGCCGGTGAAATACCACTAC76030.1377140019076731No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC73250.13267855635587342No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG72410.13115705482223608No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGC71570.12963555328859877No Hit
GGGGGATGCGTGCATTTATC71560.1296174401751031No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC70950.12851254025186648No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT69910.1266287764483155No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA69670.1261940617244191No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG68400.12389369631046745No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG67880.12295181440869196No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG65790.11916617368809435No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG65640.11889447698565912No Hit
GGGCCGCCGGTGAAATACCACTAC65530.11869523273720661No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT65380.11842353603477138No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG65300.11827863112680592No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG64970.11768089838144839No Hit
GTGGTCTAGTGGTTAGGATTCGGCG64200.11628618864228087No Hit
CTCTAGCATCACCAGTATTAGAGGC64000.1159239263723672No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT64000.1159239263723672No Hit
GGCGGGGCGCGGGACATGTGGCGTA61430.11126885620397683No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT61210.11087036770707183No Hit
CGTGATGGGGATCGGGGATT60890.11029074807521No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC59830.10837075804466766No Hit
TTGATCATCGACACTTCGAACGC59700.10813528756922379No Hit
GGGACAAGCATCAAGCACGCAGC59420.10762812039134467No Hit
GCTGAGAAGACGGTCGAACTTG57460.10407795014619095No Hit
GGGGGGGGATGCGTGCATTTATC57000.10324474692538954No Hit
CGCTGAGAAGACGGTCGAACTTG56850.1029730502229543No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC56380.10212173388865725No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG56340.10204928143467452No Hit
GTATAGTGGTGAGTATCCCCGC56100.10161456671077813No Hit
GGCATGCTCATAAGGAAAGGTTAAAA56030.10148777491630835No Hit
ACTCGCCGAATCCCGGGGCCGAGGG55390.10032853565258469No Hit
TTCACTGACCCGGTGAGGCGGGG55220.1000206127231581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)