FastQCFastQC Report
Wed 27 Sept 2023
ERR2976736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5499258
Total Bases155.3 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCTCAGATCAGACGTGGCGACCCGCTGA927941.6873912807873352No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG856151.5568463963683827No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT836661.5214052513993706No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG789531.4357027802659923No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG780961.4201188596716139No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG757901.3781859298108945No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT696191.2659707909685272No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG682441.2409674177861814No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA619261.1260791910472285No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG609681.1086586590409107No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA409820.745227810733739No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA406820.7397725293121363No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG387070.7038585932865852No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT369260.6714724059136705No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG358960.652742606366168No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG337080.6129554205312789No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC333570.6065727412680038No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG330590.6011538283892117No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC327740.5959713110386893No Hit
TCGCTTCTGGCGCCAAGCGCCCGG292270.5314717003639401No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA286050.5201610835498172No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA284120.516651519168586No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA270800.4924300696566701No Hit
AACGATGCCGACCGGCGATGCGGCGGCG266900.4853382038085865No Hit
GGCCGCCGGTGAAATACCACTAC260720.47410032408008496No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT258900.4707907866843127No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC256130.4657537435050329No Hit
ACGATGCCGACCGGCGATGCGGCGGCG233400.4244208946006898No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA219270.3987265191049411No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGA213340.3879432461615731No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG209050.3801421937286812No Hit
CGCCGCTGGTGTAGTGGTATCATGCA206810.3760689169338845No Hit
CTAAACCATTCGTAGACGACCTGCTT197530.35919391306972687No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATG197510.3591575445269162No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC194600.35386592154796154No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA190610.34661039725723No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG188770.343264491318647No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA182620.3320811644043615No Hit
CCGTCTCCGCCCCCCGGCCC180180.32764420218145796No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT176850.3215888398034789No Hit
CGCCACGCAGTTTTATCCGGTAAAGC170740.31047824997481477No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG167290.30420467633997167No Hit
CGGCCGAGGTGGGATCCCGAGGC166540.302840855984571No Hit
GCGCAGCGGAAGCGTGCTGGGC164660.2994222129603667No Hit
CGCTTCTGGCGCCAAGCGCCCGG156550.28467476885063403No Hit
GGGGGGGATGCGTGCATTTATC155120.28207441803967004No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA154180.28036509652756786No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG151590.2756553702335842No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG151160.2748734465631545No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG142330.2588167349122373No Hit
GAGGGCGCACCACCGGCCCGTCTCGC140960.25632548972970537No Hit
CCCGTCTCCGCCCCCCGGCCC139190.25310687369095974No Hit
GTCTAGGGGTATGATTCTCGCTT135770.2468878528703327No Hit
GCCGGGCGCGGTGGCGCGTGCCTGT129220.23497715509983347No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC128860.23432252132924114No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC126850.23066748277676732No Hit
CATCTGTGGGATTATGACTGAAC125740.22864902865077433No Hit
ACTGACCCGGTGAGGCGGGG122330.2224481921015526No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG121470.22088434476069319No Hit
CGCGCTACACTGACTGGCTCAGCG113530.20644603326485134No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC113350.2061187163795552No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT111520.20279099471237758No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC110430.20080890912919525No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG104640.19028021598550204No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG103610.1884072360307518No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG103270.18778897080297013No Hit
GGGGGGATGCGTGCATTTATC103070.1874252853748633No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC102750.18684338868989236No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA101050.18375206255098414No Hit
CGCTTCTGGCGCCAAGCGCCCGGC101030.18371569400817347No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC99000.180024286912889No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT98350.17884230927154174No Hit
GACCGGCTCCGGGACGGCTGGG98140.17846043957202953No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC96960.17631469554619913No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT96110.17476903247674505No Hit
ACCGCAGCTAGGAATAATGGAAT95910.1744053470486382No Hit
GGCCGAGGTGGGATCCCGAGGC92660.16849545884190195No Hit
ACCAGGAGTGGAGCCTGCGGCTT92390.1680044835139577No Hit
AAACCATTCGTAGACGACCTGCTT91240.16591329230234333No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG91150.16574963385969527No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA89480.16271286053500308No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT89010.161858199778952No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT86210.15676660378545615No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGC85960.15631199700032258No Hit
ATTGATCATCGACACTTCGAACGCA85620.15569373177254095No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT84830.15425717433151892No Hit
CGAGGGCGCACCACCGGCCCGTCTCG83850.15247511573379535No Hit
GTCTAGCGGTTAGGATTCCTGG82880.15071124140747716No Hit
GGGGGATGCGTGCATTTATC79840.1451832229002531No Hit
CGCGCGCTACACTGACTGGCTCAGCG77200.14038257524924272No Hit
CGCAGCGGAAGCGTGCTGGGC76160.13849141102308712No Hit
CACTCGCCGAATCCCGGGGCCGAGGG75350.1370184850392544No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG74750.13592742875493385No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG74230.13498184664185606No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG73780.13416355442861563No Hit
GGCGGGGCGCGGGACATGTGGCGTAC71880.1307085428616006No Hit
ATTGATCATCGACACTTCGAACGC71710.13039941024770976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG70090.12745355828004434No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT70010.1273080841088016No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC69840.12699895149491078No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA68800.12510778726875518No Hit
GAACCCCATTCGTGATGGGGATCGGGGATT68430.1244349692267575No Hit
AAGGGACAAGCATCAAGCACGCAGC68400.12438041641254147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT68280.12416220515567736No Hit
CTCTAGCATCACCAGTATTAGAGGC68230.12407128379865065No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG67840.12336209721384231No Hit
GCGCCGCTGGTGTAGTGGTATC67800.12328936012822095No Hit
GGGGGGGGATGCGTGCATTTATC66050.12010711263228603No Hit
GGGCCGCCGGTGAAATACCACTAC65140.11845234393439988No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC63590.11563378186657182No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTC63220.11496096382457414No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG62890.11436088286819784No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG62240.11317890522685059No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT62110.11294250969858116No Hit
GCCGCCGGTGAAATACCACTAC60650.11028760607340118No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC60490.1099966577309157No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC60260.10957841948859282No Hit
GTATAGTGGTGAGTATCCCCGC59920.10896015426081118No Hit
AGGGACAAGCATCAAGCACGCAGC59540.10826915194740817No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA58210.10585064385049765No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG57970.10541422133676942No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG57490.104541376309313No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT57120.10386855826731534No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG56560.10285023906861618No Hit
CGCTGAGAAGACGGTCGAACTTG56160.10212286821240246No Hit
GACCGGCGCAAGACGGACCAGAGCGAAAGC55180.10034080961467892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)