FastQCFastQC Report
Wed 27 Sept 2023
ERR2976737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13926097
Total Bases391.9 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCG11021997.914629633845004No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG9075586.516958771721898No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA7457485.355039534766992No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT5770764.143845903127057No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG4693223.3700899828573645No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG4578013.2873604140485306No Hit
CACTCGCCGAATCCCGGGGCCGAGGG2774621.9923888222234847No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG2738581.96650935290771No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA2554561.8343689549196733No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG2390691.7166977940768329No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA1626671.1680731507183957No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1383500.9934585404654297No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG962270.6909832668837508No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG944010.6778711939174343No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA933980.6706688887776668No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT887110.6370126532940277No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG881020.6326395687176386No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG804580.5777498174829602No Hit
CTCTAGCATCACCAGTATTAGAGG783000.5622537312500409No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG774570.5562003481664677No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT739370.530924062930195No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC694820.49893376442803755No Hit
AGGGACAAGCATCAAGCACGCAGC687850.4939287727207415No Hit
AAGGGACAAGCATCAAGCACGCAGC669640.48085260356868115No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT572800.4113140961175267No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT513200.3685167495242924No Hit
GAGGGCGCACCACCGGCCCGTCTCGC496140.35626636810012163No Hit
CTCTAGCATCACCAGTATTAGAGGC485160.3483818904894889No Hit
ACTCGCCGAATCCCGGGGCCGAGGG447490.3213319568289665No Hit
GAGGGCGCACCACCGGCCCGTCTCG446620.32070723046091093No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT433680.31141532333144023No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG415320.29823144273661173No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG371180.266535555511354No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC348340.25013469315918163No Hit
ATTGATCATCGACACTTCGAACGCA340240.24431827524969849No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA330670.23744628520108685No Hit
CCTCTAGCATCACCAGTATTAGAGGC323890.2325777279879639No Hit
CGCCACGCAGTTTTATCCGGTAAAGC318220.22850623545132565No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA300240.21559522384484325No Hit
CGCTTCTGGCGCCAAGCGCCCGG298640.21444630178864907No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG294430.211423200628288No Hit
TCTAGCATCACCAGTATTAGAGG288410.20710038139185732No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA281190.20191587061328095No Hit
CACTCGCCGAATCCCGGGGCCGAGG278990.2003361027860139No Hit
TGCATAATTTGTGGTAGTGGG274210.19690369814313372No Hit
GGGACAAGCATCAAGCACGCAGC270180.19400985071409454No Hit
TTGATCATCGACACTTCGAACGCA269620.19360772799442658No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG266090.19107291870794813No Hit
GCCGCGACCGGCTCCGGGACGGCTGGG258660.18573761190949625No Hit
ACGATTAAAGTCCTACGTGATCTGAGT258150.18537139300408434No Hit
TTGATCATCGACACTTCGAACGCAC252250.1811347429218682No Hit
CGCCGCGACCGGCTCCGGGACGGCTGG229000.1644394692927961No Hit
AACGATGCCGACCGGCGATGCGGCGGCG227290.16321155884523855No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG219790.1578259867068282No Hit
CGGCCGAGGTGGGATCCCGAGGC218850.1571509949988141No Hit
ACGATGCCGACCGGCGATGCGGCGGCG207080.14869923712293545No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAG205020.1472199999755854No Hit
TGCATAATTTGTGGTAGTGGGG200770.14416817576381954No Hit
CGCGACCGGCTCCGGGACGGCTGGG199960.14358653397287124No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT197410.1417554394458117No Hit
GGCGGGGCGCGGGACATGTGGCGTA195840.14062805967817113No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT194130.13940014923061356No Hit
CCGCGACCGGCTCCGGGACGGCTGGG191680.1376408623320662No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT187030.13430180760625177No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG175400.1259505804102901No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT173550.12462213928281557No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC173540.12461495851996435No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG169640.12181446100799098No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT168020.12065117742609432No Hit
ATTGATCATCGACACTTCGAACGCAC167460.12024905470642636No Hit
TTGATCATCGACACTTCGAACGC166340.11944480926709042No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCG163410.11734084575168477No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG156920.112680530661247No Hit
CGTGATGGGGATCGGGGATT155160.11141671639943339No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGT143740.10321628522334722No Hit
GGGCGTGAAACCGTTAAGAGG141940.10192374791012873No Hit
AGAGGCGGGCATAACACAGC140520.10090407958525636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)