FastQCFastQC Report
Wed 27 Sept 2023
ERR2976738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14126686
Total Bases422.1 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT7304565.170752715817425No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5244443.712434749381419No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT5142123.6400044568131547No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA4998103.53805556377483No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA4762473.3712577741163074No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT4408993.1210363138247708No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA3216262.2767264735692434No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG2661621.8841078509142202No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA2378041.6833672101156631No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2095051.483044218580352No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA2061391.4592169741721448No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG2002751.4177068846861889No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1727751.2230398552073714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1691031.1970464976711452No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1410800.9986772552316941No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1317450.9325966472249755No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1281480.9071341997691461No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1195320.8461432497331646No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1177540.833557141427225No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA1161910.8224929753517564No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG1039800.7360537354620893No Hit
CTAAACCATTCGTAGACGACCTGCTT1027350.7272406281275028No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC839300.5941237739693513No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT831080.5883049994882027No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG761510.5390577804305978No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT761000.5386967615759279No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG747850.5293881381663045No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG740500.5241852193784161No Hit
AACGATGCCGACCGGCGATGCGGCGGCG664920.4706836408765651No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG664760.4705703800594138No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG655120.46374641582604725No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC653660.4627129108695415No Hit
ACGATGCCGACCGGCGATGCGGCGGCG644010.455881867835103No Hit
GAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAA598990.4240131054091526No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT585250.41428683273628364No Hit
CGCGCTACACTGACTGGCTCAGCG564510.3996053993130448No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC532520.3769603146838544No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA527140.3731519197071415No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT517800.36654031950593363No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG494250.34986974298147494No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG493350.3492326508849988No Hit
CGCCACGCAGTTTTATCCGGTAAAGC474700.336030686885799No Hit
TCGCTTCTGGCGCCAAGCGCCCGG471600.3338362585534923No Hit
GGCCGCCGGTGAAATACCACTAC447870.31703826360973836No Hit
CGGCCGAGGTGGGATCCCGAGGC437310.3095630496777517No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG436510.3089967455919952No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG427410.3025550366165143No Hit
GAGGGCGCACCACCGGCCCGTCTCGC405260.28687549224212955No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT379680.26876791910006353No Hit
GGCGGGGCGCGGGACATGTGGCGTAC372730.2638481523550534No Hit
ATTGATCATCGACACTTCGAACGCA348930.24700060580379576No Hit
TTCACTGACCCGGTGAGGCGGGG348100.24641306531482332No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAA339450.2402899023875805No Hit
TTTTCACTGACCCGGTGAGGCGGGG319990.22651455550155214No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC318640.22555891735683797No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG292010.20670807010221645No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT288420.2041667805173839No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG286340.20269438989441685No Hit
ACCGCAGCTAGGAATAATGGAAT282790.2001814155138721No Hit
CATCTGTGGGATTATGACTGAAC280640.19865947328340133No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG276870.19599076527927356No Hit
TTTCACTGACCCGGTGAGGCGGGG271870.19245136474329508No Hit
CGGCGGGGCGCGGGACATGTGGCGTAC265750.1881191384872574No Hit
CGCGCGCTACACTGACTGGCTCAGCG260930.1847071563705741No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC258560.1830294805165203No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATT254650.1802616692973851No Hit
ATTGATCATCGACACTTCGAACGCAC242240.17147687716708646No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG239410.16947357646372263No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC234500.16599788513739175No Hit
ACTGACCCGGTGAGGCGGGG231130.16361232917614224No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG223490.15820412515716709No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA221320.1566680253245524No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAAT215710.15269681792318454No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC215320.1524207446813782No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT212260.15025463155335936No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTC209900.14858403450037752No Hit
TTGATCATCGACACTTCGAACGCAC209510.1483079612585712No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT206870.14643915777557454No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGG205760.1456534108565873No Hit
CGAGGGCGCACCACCGGCCCGTCTCG204810.1449809247547514No Hit
TTGATCATCGACACTTCGAACGCA194110.13740660760775741No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC191760.1357430893558475No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG184150.13035612174008823No Hit
GGCCGAGGTGGGATCCCGAGGC180060.1274608921016578No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG177220.12545051259722204No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA172940.12242078573842442No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGG171620.12148638399692611No Hit
CCCGGGGAGGTAGTGACGAAAAATAACAA168500.11927779806247552No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC167150.11832215991776132No Hit
GGGCCGCCGGTGAAATACCACTAC166640.11796114106309152No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGA161790.11452792254319237No Hit
GGCGGGGCGCGGGACATGTGGCGTACG159820.11313339873201683No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG159780.11310508352772901No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA159530.11292811350093009No Hit
CGCTTCTGGCGCCAAGCGCCCGG158640.11229810020552591No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC158150.111951238953No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC156290.11063458195361602No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA154740.10953736778746269No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC154670.10948781617995898No Hit
AAACCATTCGTAGACGACCTGCTT152150.10770395830982582No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAG152000.10759777629374646No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG151540.10727215144443643No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAA146450.10366904169881032No Hit
GGCGGGGCGCGGGACATGTGGCGTA145700.10313813161841355No Hit
GACCGGCGCAAGACGGACCAGAGCGAAAGC145080.1026992459519522No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC142900.10115606731826558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)