FastQCFastQC Report
Wed 27 Sept 2023
ERR2976739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2976739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14499877
Total Bases434.5 Mbp
Sequences flagged as poor quality0
Sequence length19-125
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT9076186.2594875804808545No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA6037024.163497386908868No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA5802944.002061534728881No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT5694283.927122968008625No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5232413.608589231481067No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA4280702.9522319396226604No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3976022.7421060192441633No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA2877141.984251314683566No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA2631991.815180914982934No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG2581031.780035789269109No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG2185851.5074955463415312No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1988701.3715288757277044No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1857681.2811694885411786No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1776031.2248586660424774No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1681591.159727079064188No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1473221.0160224117763206No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1430880.9868221640776677No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA1224630.8445795781578009No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1150960.7937722506197811No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1020180.7035783820786894No Hit
CTAAACCATTCGTAGACGACCTGCTT929670.641157162919382No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC855200.5897981065632487No Hit
GAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAA844460.5823911471800761No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG809590.5583426673205573No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT807890.5571702435820662No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT757560.5224596043125055No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG728350.5023146058411392No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT607120.4187070000662764No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC574780.3964033625940413No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG559050.3855549947078861No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG548610.37835493363150596No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA542170.37391351664569294No Hit
CGCGCTACACTGACTGGCTCAGCG519430.35823062499081887No Hit
AACGATGCCGACCGGCGATGCGGCGGCG497460.343078772323379No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG494470.3410166858656801No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG489520.33760286380360327No Hit
GGCGGGGCGCGGGACATGTGGCGTAC486200.33531318920843256No Hit
CGCCACGCAGTTTTATCCGGTAAAGC448330.30919572628098846No Hit
GGCCGCCGGTGAAATACCACTAC421380.2906093617207925No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT412510.28449206844995995No Hit
CGCCACGCAGTTTTATCCGGTAAAGCG399570.2755678548169754No Hit
GAGGGCGCACCACCGGCCCGTCTCGC397550.27417473955123894No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAA393130.2711264378311623No Hit
ACGATGCCGACCGGCGATGCGGCGGCG381140.2628574021696874No Hit
CGGCGGGGCGCGGGACATGTGGCGTAC341610.23559510194465788No Hit
ATTGATCATCGACACTTCGAACGCA338570.23349853243582688No Hit
CGGCCGAGGTGGGATCCCGAGGC333700.2301398832555614No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT325510.22449155948012525No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC325380.2244019035471818No Hit
TTTTCACTGACCCGGTGAGGCGGGG324600.22386396794952124No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC321570.22177429505091667No Hit
TCGCTTCTGGCGCCAAGCGCCCGG311280.21467768312793276No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGG309590.2135121559996681No Hit
TTCACTGACCCGGTGAGGCGGGG307000.2117259339510259No Hit
CATCTGTGGGATTATGACTGAAC296610.20456035592577784No Hit
TTTCACTGACCCGGTGAGGCGGGG278430.19202231853415033No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATT278400.19200162870347107No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT277270.19122231174788587No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTC269230.18567743712584595No Hit
ACCGCAGCTAGGAATAATGGAAT267590.18454639304871345No Hit
ACTGACCCGGTGAGGCGGGG259030.17864289469489983No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG244870.16887729461429224No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG239450.1651393318715738No Hit
CGCGCGCTACACTGACTGGCTCAGCG236300.1629668996502522No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG235810.16262896574915775No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG233000.16069101827553434No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGG228230.15740133519753305No Hit
ATTGATCATCGACACTTCGAACGCAC224980.15515993687394727No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG220210.151870253795946No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC215980.1489529876701713No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAAT213690.1473736639283216No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA212580.1466081401931892No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGG210810.14538744018311328No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC210150.1449322639081697No Hit
CCCGGGGAGGTAGTGACGAAAAATAACAA208180.1435736316935654No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGG204390.14095981641775307No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT202460.13962877064405443No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC201380.1388839367396013No Hit
GGCGGGGCGCGGGACATGTGGCGTACG200260.13811151639424252No Hit
GGCGGGGCGCGGGACATGTGGCGTA198260.13673219434895895No Hit
TTGATCATCGACACTTCGAACGCAC195750.1350011451821281No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC184720.12739418410238929No Hit
GCACCACCGGCCCGTCTCGC176400.12165620439400968No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGA172250.1187941111500463No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT171110.11800789758423468No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAG170750.11775961961608364No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAG169420.11684237045597008No Hit
CCGGGGAGGTAGTGACGAAAAATAACAA167670.11563546366634696No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAG167370.11542856535955444No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC164890.11371820602340281No Hit
GGGCCGCCGGTGAAATACCACTAC164340.11333889246094984No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA164130.11319406364619508No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGA164070.11315268398483656No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA162440.11202853651793047No Hit
GCACGAGCGCACGTGTTAGGACCCGAAA160950.1110009415941942No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC153450.10582848392438088No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGA151890.1047526127290597No Hit
CGAGGGCGCACCACCGGCCCGTCTCG151610.10455950764272No Hit
CGTGATGGGGATCGGGGATT150880.10405605509619151No Hit
TTGATCATCGACACTTCGAACGCA149310.10297328729064392No Hit
CGCTTCTGGCGCCAAGCGCCCGG149190.1028905279679269No Hit
CGGGGAGGTAGTGACGAAAAATAACAA146440.100993960155662No Hit
AAACCATTCGTAGACGACCTGCTT146280.10088361439203933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)