FastQCFastQC Report
Fri 23 Jun 2023
ERR3225411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3225411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35647750
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC12832573.59982607597955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT10251822.8758673408560145No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG8206972.3022406743763635No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT6430241.8038277310629705No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5299681.486680084998352No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC4412561.237822863995624No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3836061.076101577238395No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC3123250.8761422530173714No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG3067410.8604778702723174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2722440.7637059842486552No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2689120.7543589707625307No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2519500.7067767250387472No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2472390.693561304710676No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1993070.559101205545932No Hit
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA1853410.5199234173264792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1832720.5141194044504913No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG1829710.5132750313834674No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1731100.4856126964534928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA1665180.4671206457630566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG1657520.46497184254265694No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC1651640.4633223695745173No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1470110.4123990995224103No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1469540.4122392016326416No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1428970.40085839919770533No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1409630.3954330918501167No Hit
ACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGAAGAGCACA1343140.37678114326991186No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1281080.35937190986808426No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1239840.3478031572820164No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGA1212420.340111227216304No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1133540.3179836034532334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1119100.31393285691242784No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1103220.30947815780799626No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1056430.29635250471628644No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCAGATCGGAA1043980.292859998176603No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1030150.28898037043011127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC996580.2795632262905794No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA969480.2719610634612283No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA957730.2686649227510853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG952620.2672314521954401No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC859530.24111760209269867No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT859460.24109796550974466No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTAGATCGGA833050.2336893632837977No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT825480.23156580709862473No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG807270.22645749030443715No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT803650.22544199844309948No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA784760.22014292627164406No Hit
TCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGG758850.21287458535251175No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT746970.20954197670259694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCA730810.20500873126634922No Hit
CCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA702940.19719056602450366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACACG681030.19104431555988807No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA676340.18972866450196715No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA674130.18910870952584666No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA659230.18492892258277172No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA656480.18415748539529145No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT653920.18343934750440072No Hit
ACGGGCGCTGACCCCCCTTCCCGGGGGGGGAGATCGGAAGAGCACACGTC615960.17279070909103658No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAAG599760.16824624275024372No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT573840.16097509660497505No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC537930.1509015295495508No Hit
TCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT535970.1503517052268376TruSeq Adapter, Index 21 (100% over 36bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA533240.1495858784916299No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG531250.14902763849050782No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC527230.14789993758371847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG500530.14040998379981906No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA496550.13929350379757488No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT492990.13829484329305497No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC487220.13667622781241454No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA475820.13347827001704177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC466130.1307600058909749No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAGATCGGAAGAG459290.1288412312137512No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC445950.12509905954793782No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAAGATCGGAAGAGCACACGTCTG429060.12036103260374077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG427330.11987572848216226No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTGAGATCGGAAGAGCACAC427180.1198336500901179No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGC427040.11979437692420981No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT423160.11870594918332854No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC419890.11778864023676107No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT419110.11756983259813031No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC416650.11687974696860251No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC412640.11575485128794945No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCT407910.11442797932548338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT400200.11226514997440232No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAGATCGGAAGAGC399560.11208561550167961No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT399270.11200426394372717No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAGATCGGAAGAG398930.11190888625509324No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG394060.11054274112671908No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG392510.11010793107559384No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTAGATCGGAAG376940.10574019398138733No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC371720.10427586593824295No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA370200.10384947156552658No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC364890.10235989648715556No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTG357630.10032330231220764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)