FastQCFastQC Report
Fri 23 Jun 2023
ERR3225412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3225412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36857993
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT15098624.0964303183844No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT9899952.685970991421047No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG8216462.2292206740611187No Hit
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA6079291.6493817229820407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5900231.6008006730046316No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5795001.5722505563447255No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT4665391.2657742921596409No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA4133241.1213958394316261Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2973860.8068426297655437No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2925740.7937871169490971No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2879300.7811874075726261No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG2642610.7169706717346221Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2578770.6996501410155458No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2392610.6491427788810965No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2199050.5966277111181827No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG2163140.5868849125887022No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG2155100.5847035675545329No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2025110.5494357763864137No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1825490.49527656050073043No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1807140.49029799316528166No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGA1789490.48550934392982276No Hit
ACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGAAGAGCACA1747000.4739813152604375No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCAGATCGGAA1646710.44677147776331716No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT1494330.40542902051123625No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1414950.3838923079723847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1386370.3761382232613697No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1381720.3748766244542941No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1340500.36369316148060477No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1249490.33900109536620726No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1199530.3254463692583587No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1191020.32313750778562467No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGC1181870.3206550069071857No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1170820.31765701404305985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1153240.31288735661759987No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA1134410.3077785597278723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1115330.30260193494529125No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1058830.2872728311603944No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA1022900.27752460639948573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1021920.2772587210594999No Hit
TCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGG958660.2600955510518438No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC927310.25158993328801166No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT911090.2471892595996749No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT897400.24347500418701581Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGAGCA883850.23979873239435473No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA880300.23883557631583469No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTAGATCGGA878270.23828481382586406No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG864010.23441591081749896No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA842050.22845790870924523No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAAG821900.22299098054525107No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGAGATCGGAAGAG804290.2182131837726487No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG784360.21280594415436566No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC734800.19935974267508272No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA726420.19708615170663255No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGATCGGAAGAGCACACGT684440.1856964919386685No Hit
TCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAAC678540.1840957536673253Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCA675840.1833632124245072No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA666460.18081830988464295No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT648560.17596183275633048No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG647210.17559556213492145No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAG645300.17507735703352054No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG645190.17504751276066496No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT636750.17275764309792993No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT626760.17004724049950307No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA596650.16187804908422443No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTAGATCGGAAG592680.16080094214571045No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC590820.1602963026228802No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC587460.1593846957429288No Hit
TCGCCCGTCACGTTGAACGCACGTTCAAGATCGGAAGAGCACACGTCTGA549660.14912911834347573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT544090.14761791289069917No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTAGATCGGAA519160.1408541154153456No Hit
TCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT497550.13499107235708682TruSeq Adapter, Index 22 (100% over 36bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTAGATCGGAAGA490610.13310817005147296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG487370.13222912056009128No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC484290.13139348092013584No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAG482870.1310082184887278No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT480740.13043032484161576No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA480340.13032180021305012No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTAGATCGGAAG479650.13013459522877438No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACACG477180.12946445564738154No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA474460.1287264881731352No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA471640.12796138954174743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA469770.12745403690320306No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTG462650.12552229851473465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTTAGATCGGAAGAGCACACGTCTGAA454080.12319715834771577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT451340.12245376464204115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC449070.12183788737493113No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA447320.12136309212495645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA447230.12133867408352919No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA445490.12086659194926865No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT445450.12085573948641208No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAGATCGGAAGAGCACACG442840.12014761628502127No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC440670.11955887017505266No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAAGATCGGAAGAGCACACGTCTG433180.11752674650516103Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA432170.1172527218180328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTGAGATCGGAAGAGCACAC429530.11653645926949956No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGATCGGAAGAGCACACG421700.11441208966532714No Hit
ACGGGCGCTGACCCCCCTTCCCGGGGGGGGAGATCGGAAGAGCACACGTC420340.11404310592820396No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT416910.11311250723825358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT415710.11278693335255666No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC413560.11220361347401633No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACG413120.11208423638259413No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC412050.11179393300118105No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG404790.10982421099271465No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC400810.10874439093848653No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGAGC396910.10768627580997152No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAGATCGGAAGAGCACACGT393490.10675839023573529No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGA380050.10311196271592976No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT379630.10299801185593582No Hit
CAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGA373440.10131859322888254No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC371660.10083565863176544No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG370350.10048024047321297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)