FastQCFastQC Report
Fri 23 Jun 2023
ERR3225416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3225416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38416174
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT19103704.972827330488455No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT11404222.9685985907914723No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG10459092.7225746114123703No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8346182.1725692933398313No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7285131.896370523519599No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG4842951.2606539110323687No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG4801211.2497886957717341Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC4745431.2352687698676084No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3952451.0288505044776193No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3601810.9375764489196661No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3530550.9190269702547682No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCAGATCGGAA3005920.7824620952622716No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2860640.7446446905410206No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2683000.6984037504619799No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2655020.6911203598775869No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG2559230.6661855498676156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGAAGAGCACA2376440.6186040286052432No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGA2318100.6034177167148399No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2103870.5476521425584963No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2084980.5427349428394405No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1913620.498128730882987No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1876560.48848175250351583No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1788890.4656606355437686No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1776630.46246927140636124No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1740460.453053966279932No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA1647170.4287699238346848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG1640200.4269555838642338No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1632810.4250319149429092No Hit
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA1480180.38530125358136913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1464080.38111031046454547No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1359370.35385356178363836No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1341780.3492747611982391No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1226480.31926136111316034No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1177870.30660783658466356No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC1176270.30619134534324005No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG1139240.29655217617454566No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1119290.29135905100804677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1090920.2839741406835569No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA1053580.27425427633683663No Hit
TCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCA1048330.2728876644509159No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA997940.2597707934163355No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT978660.2547520739571827No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT972550.25316159802899685No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT914460.23804036289506603No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC911640.23730629708205714No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTAGATCGGA897020.2335006083635502No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC886580.23078300301326207No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA877760.2284870950449152No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC820630.21361575465583846No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA785260.20440869515012086No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTAGATCGGAAG777960.20250845386112631No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC775850.20195920603649908No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAA771930.20093880249501161No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA750330.19531617073579474Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT741260.19295518601097547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAAG732590.19069832409651205No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGAGCA692960.1803823566605045No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC676900.17620182582471644No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA670780.17460874682627167No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA659430.17165426208242393No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA629660.1639049219216885No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAGATCGGAAGAG628240.1635352859449252No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTC623810.16238212582023395No Hit
GGCTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCT621800.16185890869819572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG610610.15894607307849032No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA597660.15557509709321912No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGATCGGAAGAGCACACGT587690.15297983604509913No Hit
ACGGGCGCTGACCCCCCTTCCCGGGGGGGGAGATCGGAAGAGCACACGTC581160.1512800311660396No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA579160.15075941711426025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA575870.14990300699908324No Hit
TCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGG562390.14639406829009052No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT559940.14575631607666084No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT542310.141167103210226No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG539730.14049551108343064No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGC539640.14047208345110057No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT533650.13891284436602147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC531770.13842346715734888No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC526910.13715837501152509No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC510140.13279302618735536No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT505130.1314888879876481No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTGAGATCGGAAGAGCACAC472940.12310960482425971No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT466880.1215321442473683No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC445540.1159771923148828No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT440160.11457674051559637No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT438230.11407434795562932No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC438190.11406393567459372No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAAGATCGGAAGAGCACACGTCTG432700.11263485010245945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC420670.10950335658100674No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG420120.10936018771676742No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC412160.10728814379068566No Hit
CAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGA412130.10728033457990896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG408890.10643693981602645No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC407920.10618444200091347No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTCT405120.10545558232842239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAG401700.10456533229987973No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT396500.10321173576525347No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTAGATCGGAA396440.10319611734370007No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG392300.10211844625651685No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG391510.10191280370606401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)