FastQCFastQC Report
Fri 23 Jun 2023
ERR3225418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3225418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40468935
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT18341864.532330786565053No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT11673732.8846150757364875No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG10568472.611501884099495No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7813321.9306957299469334No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC6433361.589703311935439No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC5075501.2541718728204734No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA4263401.0534994311068477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA4212371.0408897590213333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG4084021.0091740738914925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3943920.974554927131144No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3938460.9732057441096487No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3544250.8757952241639173No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG3012090.7442968291604412No Hit
ACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGAGATCGGAAGAGCACA2975400.7352306157797333No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2936360.7255837100729239No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG2892730.7148026010568354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2754930.6807517914667138No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2187710.5405899611640386No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2112550.5220176908534905No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGA2004960.49543186644274184No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1966780.48599746941697375No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1955840.4832941613116332No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG1925360.47576245829053815No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCAGATCGGAA1897260.46881886068906925No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1742360.4305425877898689No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1672000.4131564124432729No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1575210.38923930170141613No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1553620.38390434539480717No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1521780.3760365821339257No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1506600.3722855568104276No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1454100.3593126431422028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT1370070.33854856817951845No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1304150.3222595306745779No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1290270.31882973940381676No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG1236300.3054935841528817No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1188370.2936499317315862No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1165750.2880604592139625No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1164770.2878182981588223No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGATCGGAAGAGCACACGT1156500.28577475537718006No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC1122520.27737819144486997No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1112240.27483797139707283No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1060390.26202567475521654No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1037270.25631265067884784No Hit
TCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGATCGGAAGAGCA993070.24539069288579005No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC859980.21250373897904654No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA798080.1972080560064158No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGAGCA779530.19262429317697638No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA777250.19206089806909918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA769160.1900618338486051No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAAG752010.18582401538365168No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA746380.184432824832183No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT740310.18293290890901873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA717200.17722235586382493No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC706140.1744893953843856No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT671250.16586796761515962No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA668520.16519337610441193No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC660450.16319925394626766No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT659350.16292744051702868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA658770.16278412070888448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC648700.16029579231576024No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT648690.16029332128458534No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG648040.16013270425821682No Hit
ACGGGCGCTGACCCCCCTTCCCGGGGGGGGAGATCGGAAGAGCACACGTC635010.1569129506373222No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGC634120.1566930288627561No Hit
TCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAAC619600.15310509159680133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA598050.14778001941489194No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC592020.14628998761642725No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA591240.1460972471847851No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC586260.1448666736596849No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA576530.14246236032650722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTAGATCGGAAG570190.14089572656162064No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC565400.13971210262884357No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA560290.13844940569846972No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT559360.138219599799204No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT550820.13610933917583945No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT546010.13492077318071255No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC532340.13154287356462432No Hit
GGCTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCT524230.12953886728178043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC515610.12740883840901668No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAAGATCGGAAGAGCACACGTCTG513940.1269961762028084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTC507920.12550861543551864No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT506570.12517502622690713No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA494720.1222468542846507No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG490500.1212040791288429No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG465730.1150833349086157No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAGATCGGAAGAGCACACG460730.11384781932116572No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC460550.11380334076001752No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC459370.11351175908137934No Hit
TCGCCCGTCACGTTGAACGCACGTTCAAGATCGGAAGAGCACACGTCTGA448800.11089987912951008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG439670.10864382766682641No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT437700.10815703452537112No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT435170.10753186363812145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTCGGGCTGGGGCGCGAAGCGGGGAGATCGGAAGAGCACACGTCTGA429810.10620739092837507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA427950.10574777912984368No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC425200.10506824555674618No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTAGATCGGA416660.10295798493338162No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC409830.10127027064092495Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG408960.10105529092870864No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACG408510.10094409452583815No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGC408460.10093173936996365No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT404810.10002981299112516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)