FastQCFastQC Report
Fri 23 Jun 2023
ERR3276523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3276523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46244710
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length1-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCT3506000.7581407689657909RNA PCR Primer, Index 3 (100% over 50bp)
A3227780.6979782119944098No Hit
C2385570.5158579219115008No Hit
GGATACACAATTCTGAGGGATCATAGCATCTCCATTTTGGAAAAAAGTTA1945930.4207897508709645No Hit
T1596150.3451529915529798No Hit
GGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGC600790.12991540005332503RNA PCR Primer, Index 3 (100% over 50bp)
TACACA505640.10934007370789005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)