FastQCFastQC Report
Wed 27 Sept 2023
ERR3403465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27588151
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7466712.7064916383848994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC6719972.43581746380901Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4845851.7564968380809571No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA4443261.6105682472159877No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4094091.4840030417406371No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3319641.2032847000148723No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2668610.9673029555333374No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2529120.916741393796199Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2374110.860554228516438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2271730.8234440938067941Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2248560.8150455606829178No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2032020.7365553421829538No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC1923410.6971869916182495No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT1837990.6662244236665226No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1537750.5573950932775451No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1378970.49984139930218596No Hit
GTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1207600.4377241519375474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1167320.423123680887494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1103000.39980932393765717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1064440.3858323089503171No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG988720.35838574321272926No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT965380.34992558943149177No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC878760.31852805213368596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC867960.3146133280189745No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT817340.29626487110354005No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG806300.2922631531196129No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC797720.28915312229514767Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC781090.2831251721074022No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC753720.27320424627224926No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT688780.24966515516027152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC654650.2372939020088733No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG647190.23458984257408189No Hit
GTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA646380.23429623826547855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC645470.2339663865113686No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG635450.23033439247160856No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA622750.22573096689227196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG612150.22188873766857375TruSeq Adapter, Index 2 (100% over 49bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG604680.21918105348923167No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC589960.21384542951066202No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT586350.21253689672787424No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG577280.20924925342042677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG556190.2016046671630875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC552140.20013664562007075No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG546330.19803066903613803No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG535990.1942826831707569No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA523410.18972275452602821No Hit
ACGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC521990.1892080407998347No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG498960.18086025409966763No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC495420.17957709452873447No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC481970.17470181310809849No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT469920.1703339959245547No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT447760.16230156200029497No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACG438990.15912266102936728No Hit
CGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACG403510.14626206736363012No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT397960.14425033413801455No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA395260.14327165310933668No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC387110.14031748630054983No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT372610.13506160670209466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT364020.13194795113307883No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT353280.12805497548567135Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC350350.12699292533232834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG348860.1264528383942802No Hit
GTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC319160.11568734707882382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT315630.11440781225244127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT313240.11354149830483384No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG311360.11286004632931No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT302980.10982251039585798No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC298170.10807900826699114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC297000.10765491315456407Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA296280.10739393154691665No Hit
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC293660.10644424847464405No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG278210.10084401814387634No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC276820.10034017865133477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)