FastQCFastQC Report
Wed 27 Sept 2023
ERR3403466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29148173
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7750802.6591031966223064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC6989352.39786898479023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT5079531.7426581075939134No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA4606581.5804009397089827No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4292081.4725039541929437No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3449951.1835904775232398No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2786090.9558369232953297No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2634280.9037547567732633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2501560.8582218858108192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2385190.818298285796506No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2356540.8084691963369368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2121800.7279358469568573No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC2011020.6899300343798563No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT1942190.6663162044495894No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1612760.5532971140249511No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1448130.4968167301600687No Hit
GTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1251880.4294883250487089Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1215990.4171753749368785Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1151470.3950401968589935Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1110280.3809089509658118No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1020780.3502037674882745No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1015790.34849182485639835No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC915510.31408829637452745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC910470.3123592000088651No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT847040.2905979733275221No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG845240.2899804389112141No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC824000.2826935327987795No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC821860.28195935299272445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC795330.2728575818456958No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT728280.2498544248382223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC684280.23475913910624863No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG680940.23361326968932153No Hit
GTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA674120.2312735004008656Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC667610.22904008426188494No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG663140.22750654046138674No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA644050.2209572449017645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG636220.21827097019082464No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG630050.21615419944159106TruSeq Adapter, Index 2 (100% over 49bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC613160.21035966816856755No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT612130.21000630125256906No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG608920.20890503154348644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG578190.19836234675840572No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC570350.19567264130070863No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG568890.19517175227414768No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG559070.19180275895851173No Hit
ACGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC554860.19035841457370245No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA548440.1881558751555372No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC521880.17904381176823672No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG509680.1748583007243713No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC504980.17324584974845592No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT492700.16903289273053237No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT468240.16064128616225792No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACG456410.15658271274841137No Hit
CGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACG428970.14716874364647142No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA415490.14254409701767587No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT414090.1420637924716585No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC407720.1398784067872796No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT386200.13249543976564157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT385030.13209404239504136No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT372660.1278502086563024Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC365080.12524970261429422Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG363630.12475224433449053No Hit
GTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC330430.11336216510036494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT327580.11238440227454394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG326980.11217855746910793No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT326440.11199329714421552No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT318420.10924183824488759No Hit
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA314990.1080650921071451No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC311410.10683688476804361Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC306890.10528618723375904No Hit
GTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC304650.10451769996013129Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)