FastQCFastQC Report
Wed 27 Sept 2023
ERR3403471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22702884
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG894940.39419661396323036TruSeq Adapter, Index 6 (100% over 49bp)
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT360020.15857897172887817No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGAGATCGGAAGAGC301820.13294346216101885No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAG280040.12334996734335603No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAAGATCGGAAGAGCA270490.11914345331632756No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)