FastQCFastQC Report
Wed 27 Sept 2023
ERR3403473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11920376
Total Bases607.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4992064.1878376990792905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4803854.0299483841784856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2645942.2196783054494253No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2097821.7598605950013657No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1788041.499986242044714No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1200321.006948102979302No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC1167960.9798013082808797No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1107610.9291737106279199Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1090950.91519764141668No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1017700.8537482374717039Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC917970.7700847691381547No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT801270.6721851726824725No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA751360.6303156880286326No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC743140.6234199323913944Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT726720.609645199111169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC715140.5999307404397312No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT663310.5564505683377773No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC628580.5273155813205892Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT576210.4833824033738533No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA566640.4753541331246598No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG555300.4658410103842362No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC525100.4405062390649422Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC488760.4100206235105336No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA479140.40195040827571205No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT477650.40070044770399865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG476300.39956793309204336TruSeq Adapter, Index 9 (100% over 49bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA446110.37424155076987503No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC445930.37409054882161435No Hit
GTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC442330.3710705098564005Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAAC435640.3654582707793781Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG416400.3493178403097352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC413890.3472122020312111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT411830.34548406862333875No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG386990.32464579976336316No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC385810.3236558981025431No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG327170.27446281895805974No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT324960.2726088505933034No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC314960.2642198534677094No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT300980.252492035486129Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG295250.2476851401331636No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG293770.24644356855857566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA289200.24260979687217918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC261470.21934710784290698No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT258650.21698141065348947No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG256580.2152448882484915No Hit
GTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA253740.2128624130648228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC252100.21148661753622539No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC252010.21141111656209502No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC244820.2053794276287929No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC242830.20371001720079973No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT235280.19737632437097624No Hit
TAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACT225350.18904605022526136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG220920.1853297244986232No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC212610.17835846788725457No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG207710.1742478592957135No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC205370.1722848339683245No Hit
TTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT198360.1664041469832831Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC197090.16533874434833262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC189390.15887921656162524No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGA188130.1578222029238004No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCACA187320.15714269415662727No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT185250.15540617175162932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC184570.15483571994708892Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG184100.154441437082186No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA182030.15270491467718805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACAC181450.1522183528439036No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG179260.1503811624733985No Hit
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC177060.1485355831057678Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC169530.1422186682701955Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA168070.14099387468985877No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGT162410.13624570231677258No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG160690.13480279481117038No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT160450.13460145888015612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC155960.1308347991707644No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG137880.11566749236769042No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG137180.11508026256889883No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGA135100.11333535116677527No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGG133450.11195116664105226No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC127800.10721138326509162No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT127610.10705199231970536No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG126330.10597820068762931No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT124980.10484568607567411No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACG122060.10239609891500066No Hit
CGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAAC121240.10170820115070196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC120340.10095319140939849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)