FastQCFastQC Report
Wed 27 Sept 2023
ERR3403475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23462367
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1137320.48474222570979303TruSeq Adapter, Index 9 (100% over 49bp)
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGAC441750.18828023617565953No Hit
ACGAAGGTGGTTTTCCCAGGGCGAGGCTTAGATCGGAAGAGCACACGTCT334680.14264545431413633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACTATACTTTCAGGGATCATTTCTATAGTGTAGATCGGAAGAGCACA282580.12043968112850677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)