FastQCFastQC Report
Wed 27 Sept 2023
ERR3403477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16458018
Total Bases839.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT7354444.468606122559836No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3720112.2603633074164824No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2993821.819064725776822No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT2941121.7870438591086728No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2657251.6145625797711487No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2248751.3663552925996314No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2173571.320675430054822No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2094691.2727474231708824TruSeq Adapter, Index 5 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1566710.9519433020428097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1291190.7845355376327818No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1235180.7505034931909784No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1076190.6539001233319832No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT958630.5824698940054629No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG943050.5730033835179911No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC928730.5643024573189797No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC914500.5556562157120013No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG868400.5276455524595975No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT844490.5131176791761924No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC826510.5021929129011768No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG822510.49976248658860384No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT788610.4791646235895477No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT782560.47548860379178104No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT744040.45208359840170304No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG731150.4442515496094365No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA723000.4392995559975691No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG587700.3570903859747875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGA567420.3447681245700424No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC546490.3320509188895042No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG532060.3232831559668971No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG481380.29248965458659726No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC475820.2891113620121208No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG473130.2874769003169154No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT471400.2864257409367276No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC456140.27715366455426166No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC446690.27141178239080793No Hit
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGAGATCGGAAGAGCAC442820.2690603449333936No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG438870.2666602989497277No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGT401250.2438021394799787No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC397880.24175450531163595No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC390340.23717315171243583No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTAGATCGGAAGAGCACACG369780.22468076046581065No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA342900.20834829564532012No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCAGATCGGAAGAGCACA340230.20672598608167764No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT339660.206379650332136No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC327700.1991126756575427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT325900.19801898381688487No Hit
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC314570.19113480128652185No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGA314290.19096467144464174No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG305680.18573317880682838No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACA286180.173884850533035No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC285540.17349598232302335No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA281480.17102909961576174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC281090.1707921330502859No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG274570.1668305381607919No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG269190.16356161477038123No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC268040.1628628672055165No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACAC263200.15992205136730317No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACG255510.15524955678138158No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC245110.1489304483686918No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCAGATCGGAAGAGCACA239480.14550962333374529No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC229560.13948216607856426No Hit
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA219960.1336491429283891No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT219210.13319343799478164No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC212600.12917715851325476No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT210570.12794371715962397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA209930.12755484894961228No Hit
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGCAGATCGGAAGAGCA207740.12622419054347855No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC206190.12528240034735652No Hit
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC198310.12049446051158773No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA197280.11986862573610019No Hit
TCTGAAGGTCCTGAGTTCGAGCCTCAGAGAGGGAGATCGGAAGAGCACAC194700.1183010007644906No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACA193400.11751111221290438No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC192520.11697641842413832No Hit
CGCATCGACCTGGTATTGCAGTACCTCCAGGAACGGTGCAGATCGGAAGA192080.11670907152975529No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAGATCGGAAGAGC179150.10885271847436306No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC175730.10677470397711317No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA174020.10573569672848822No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA170400.10353616091560965No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG170390.10353008484982822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)