FastQCFastQC Report
Wed 27 Sept 2023
ERR3403478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17334635
Total Bases884 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT7665114.421846782467586No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3886332.241945100084311No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA3120241.800003288214606No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT3046431.7574237934632024No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2757571.5907863072975001No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2322141.339595555372236No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2267461.3080517703430157No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2142341.2358725753383326TruSeq Adapter, Index 5 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1615390.9318857881922521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1345450.776162867000084No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1277940.7372177147081551No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1123050.6478648093830646No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1011590.5835657918381322No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG979700.5651690964361235No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC963200.5556505804708319No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC954450.5506028826104501No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG908780.5242567841780343No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT878410.5067369460043434No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG854770.49309950858498025No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC849820.4902439537953929No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT823170.4748701083120585No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT809630.46705915642296475No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT783450.4519564444247023No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG765110.441376469709342No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA749730.4325040590701794No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG613200.3537426660555587No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGA584290.33706507232485716No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC573200.33066747583667033No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG556290.32091243917163526No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG500740.28886676875515405No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG497080.2867553888501258No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC493080.28444786982823694No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT482330.2782464124569107No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC477660.27555238399885545No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC471090.27176228400540303No Hit
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGAGATCGGAAGAGCAC467320.26958744732727286No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG453590.2616668882846394No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC415390.23963008162560098No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGT411820.2375706208985652No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC405570.2339651224268639No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTAGATCGGAAGAGCACACG379030.2186547337166315No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT359030.2071171386071873No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA358330.20671332277835672No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCAGATCGGAAGAGCACA350670.20229442385143961No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT343580.19820434638514167No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC335350.19345662599760538Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC326770.18850699769565382No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGA325340.18768205964532855No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG312690.1803845307386051No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACA302190.1743272933061469No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC301070.17368118798001805No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA299810.17295431948812306No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC290270.16745088662091817No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG287190.16567409697406377No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG281210.16222435603633995No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC278610.1607244686721122No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACAC270700.15616134980632704No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACG268450.15486337035651457No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC258940.14937724388197385No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCAGATCGGAAGAGCACA248350.14326808727152315No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC240730.13887226353482493No Hit
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA236620.13650128773983417No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT225740.13022483600029652No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC220280.12707507253541825No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT219070.12637704803129687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGCAGATCGGAAGAGCA219040.1263597416386327No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA218620.12611745214133438No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC214560.12377532033411721No Hit
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC205820.11873339127129012No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACA204370.1178969156258554No Hit
CGCATCGACCTGGTATTGCAGTACCTCCAGGAACGGTGCAGATCGGAAGA202740.11695660162443572No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA202240.11666816174669961No Hit
TCTGAAGGTCCTGAGTTCGAGCCTCAGAGAGGGAGATCGGAAGAGCACAC201900.11647202262983905No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC198650.11459716342455437No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAGATCGGAAGAGC185590.10706311381808731No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA183120.10563822082207096No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC180710.10424794061138294No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA177200.10222309266967547No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACG177110.10217117349168299No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC173930.10033669586928136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)