FastQCFastQC Report
Wed 27 Sept 2023
ERR3403486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15392587
Total Bases785 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4997423.246640736868988No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2941001.9106599819770385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC2128511.3828149875001519No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1924971.250582504422421No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1889131.2272985691099227No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC1727901.122553343372365No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1519270.9870140737226302TruSeq Adapter, Index 12 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1327940.8627139804374665No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA1241030.8062517366314057No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1219460.7922384976612443No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1146380.7447610983131037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1095370.7116217696219616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1070580.695516614588568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1064220.6913847555319973No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC976430.634350807957103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC892240.5796556485274372No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG831520.5402080884779148No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG808350.5251553881098739No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT782200.5081666908882828No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT734210.4769893455856381No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT715660.46493809000397396No Hit
TTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAAC707580.45968880994468314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC681830.4429599780725618No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC663720.4311945743753145No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG570890.37088632339710015No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC544960.35404055211771746No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC520950.33844213451578997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT520950.33844213451578997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC518430.33680498281412996No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC512040.33265363385634916Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC508310.33023038947254285No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC491190.31910815251523345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC475290.30877850487380715Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT466640.30315891669152173No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC464770.30194404618275017No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT457390.2971495304850315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC421830.2740475009171623No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC420980.27349528704953885No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT418360.27179316901051137Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG395300.25681193161357474No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA394030.25598685912900804No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC389620.25312184365110296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCA381750.24800899290028372No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA376660.24470220632827996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC375370.24386414057623973No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG371220.24116803757549005No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA364500.23680229970439665No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA351390.2282852128755225No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT350980.2280188508923159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGT347080.22548516373498492No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT313110.20341609893125828No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT303180.19696494163066935No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG296390.19255372732341874No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGA290140.1884933312379524No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC287510.18678471656518816No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC287310.18665478389045326No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC285700.18560882585883712No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT278170.18071686065506729Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC277340.18017764005491735No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG276870.17987229826929027No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT273660.17778687883979474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG265720.17262855165281835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC260110.16898394012650375No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT256860.16687253416206124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT253680.164806604633776No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC249370.16200655549323842No Hit
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC240170.15602965245543196No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG233010.1513780626999217No Hit
CATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT230810.14994880327783758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGT228620.14852604048949017No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG225630.14658354700220308No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT221740.14405635647860882No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTAGATCGGAAGAGCACACG218850.1421788293286892No Hit
GTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA215520.14001545029435272Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC209840.13632536233188092No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC207090.13453878805427574No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC206690.1342789227048059No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG205510.13351231992386983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA193100.12544999745656787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG187680.12192882197125149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGAGATCGGAAGAGCAC181040.11761505717005204No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT180800.11745913796037014No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC177980.11562708724660772No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCAGATCGGAAGAGCACA177870.11555562427550353No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT169570.11016341827400422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC168030.10916293667854533No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT166630.10825340795540088No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA166590.10822742142045388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT165560.10755826814556903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG161980.10523247326781392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT161700.10505056752318502No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACACGTC160020.10395913305541167No Hit
TAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACT159220.10343940235647198Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG158970.10327698651305332No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC156750.1018347338234957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)